PMID: 6166623Jul 1, 1981Paper

Immunocytochemical localization of P0 protein in Golgi complex membranes and myelin of developing rat Schwann cells

The Journal of Cell Biology
B D TrappH D Webster

Abstract

P0 protein, the dominant protein in peripheral nervous system myelin, was studied immunocytochemically in both developing and mature Schwann cells. Trigeminal and sciatic nerves from newborn, 7-d, and adult rats were processed for transmission electron microscopy. Alternating 1-micrometer-thick Epon sections were stained with paraphenylenediamine (PD) or with P0 antiserum according to the peroxidase-antiperoxidase method. To localize P0 in Schwann cell cytoplasm and myelin membranes, the distribution of immunostaining observed in 1-micrometer sections was mapped on electron micrographs of identical areas found in adjacent thin sections. The first P0 staining was observed around axons and/or in cytoplasm of Schwann cells that had established a 1:1 relationship with axons. In newborn nerves, staining of newly formed myelin sheaths was detected more readily with P0 antiserum than with PD. Myelin sheaths with as few as three lamellae could be identified with the light microscope. Very thin sheaths often stained less intensely and part of their circumference frequently was unstained. Schmidt-Lanterman clefts found in more mature sheaths also were unstained. As myelination progressed, intensely stained myelin rings became much more n...Continue Reading

References

Apr 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G BaskinJ A Parsons
Nov 1, 1977·The Journal of Cell Biology·R M Gould
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B D TrappH D Webster
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerH D Webster
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerH D Webster
Sep 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G BaskinJ A Parsons
Aug 18, 1978·Brain Research·R G Peterson, R W Gruener
Oct 1, 1972·Journal of Ultrastructure Research·R G Peterson, D C Pease
Apr 1, 1973·Journal of Neurochemistry·S GreenfieldP Morell
Mar 1, 1980·Journal of Neurochemistry·A T CampagnoniY T Yu
Feb 1, 1980·Journal of Neurocytology·J P BrockesJ Winter

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Citations

Jan 1, 1991·Acta Neuropathologica·P Baron, B Kreider
Jan 1, 1989·Acta Neuropathologica·D Soffer, J R Martin
Jan 1, 1988·Acta Neuropathologica·J P HammangS A Gilmore
Sep 1, 1991·Journal of Neurocytology·S MorrisonD Kirkham
Jun 1, 1989·Journal of Neurocytology·I R GriffithsJ A Barrie
Dec 1, 1994·Journal of Neurocytology·G J KiddB D Trapp
Jun 19, 1992·Brain Research. Developmental Brain Research·A KondoH Nagara
Mar 29, 1996·Brain Research. Developmental Brain Research·R E CoggeshallM Fitzgerald
Apr 1, 1987·Brain Research·A C LeBlancC Mezei
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A C LeBlanc, C Mezei
Sep 1, 1988·Neuron·G Lemke
Jan 1, 1986·Acta histochemica·S DolapchievaK Ichev
Mar 23, 2000·Progress in Neurobiology·B GarbayC Cassagne
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S Billings-GagliardiM K Wolf
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D TrislerM P Daniels
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I D DuncanB D Trapp
Jan 1, 1990·Annals of the New York Academy of Sciences·A Messing
Jan 1, 1991·Annals of the New York Academy of Sciences·K R JessenL Morgan
Jan 1, 1987·Journal of Veterinary Internal Medicine·I D Duncan
Jun 1, 1990·Journal of Neurochemistry·L RutkowskiG I Tennekoon
Jun 1, 1992·Journal of Neurochemistry·E WongH C Powell
Nov 26, 2003·Experimental Neurology·Yuki HaraNaoyuki Ochiai

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