Immunocytochemical localization of polyamines in the gastrointestinal tracts of rats and mice

Histochemistry and Cell Biology
K FujiwaraT Kitagawa

Abstract

An immunocytochemical method using a recently produced monoclonal antibody (ASPM-29) with an antibody specificity to spermine (Spm) and spermidine (Spd) fixed in situ, was used to demonstrate an immunocytochemical localization of polyamine (PA) pools in the gastrointestinal tracts of rats and mice. High PA immunoreactivity was always found in the cytoplasm of cells not only at the cell proliferative zone or the precursor cell zone but also at the neighboring non-proliferative premature cell zone of the epithelium, and a gradient of decreasing PA levels was noticed from these cells to the fully mature differentiated gastric surface mucous cells and absorptive cells of the small and large intestines. Also, strong staining for PAs was seen in the cytoplasm of fully differentiated gastric chief cells and neurons of both the myenteric and submucous plexuses, whereas the nuclei of the cells remained virtually unstained. These results may suggest that PAs are closely associated with the high biosynthetic activity in the cells of the gastrointestinal mucosa of normal rats and mice. This seems to be consistent with the PA immunocytochemical results previously obtained for neoplastic cells and active protein- or peptide-secreting cells, ...Continue Reading

References

Apr 6, 1978·Biochimica Et Biophysica Acta·J JänneA Raina
Jan 1, 1986·Cell and Tissue Research·A R GreenfieldM K Haddox
May 15, 1989·The Biochemical Journal·F Schuber
Mar 1, 1986·The Biochemical Journal·A E Pegg
Dec 1, 1969·The American Journal of Anatomy·H ChengC P Leblond
Jan 1, 1981·Differentiation; Research in Biological Diversity·O Heby
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Jan 1, 1993·Life Sciences·Y ElitsurG D Luk
Oct 1, 1995·Histochemistry and Cell Biology·K Fujiwara, Y Masuyama

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Citations

Oct 11, 2005·Histochemistry and Cell Biology·Masashi ShinKunio Fujiwara
Apr 20, 2007·Histochemistry and Cell Biology·Masashi ShinKunio Fujiwara
Feb 28, 2008·Histochemistry and Cell Biology·Masashi ShinKunio Fujiwara
Apr 19, 2003·Journal of Gastroenterology and Hepatology·Katsuhiro UdaTadao Bamba
Jun 2, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kunio Fujiwara
Apr 18, 2013·PloS One·John Bradley GoforthEce Karatan
Sep 19, 2014·Nucleic Acids Research·Helen L Lightfoot, Jonathan Hall
Jul 9, 2004·The Journal of Cell Biology·Rosa CaroppoSilvana Curci
Aug 29, 1997·Brain Research·K FujiwaraT Kitagawa
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K FujiwaraD Tsuru
Apr 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kunio FujiwaraKazuhiro Tsukamoto
Jun 23, 2004·The Journal of Biological Chemistry·Denis SouletRichard Poulin
Nov 22, 2017·Histochemistry and Cell Biology·Yutaro YamamotoMasashi Shin
Jan 17, 2020·Nutrients·Anna F BekebredeVincent C J de Boer

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