Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster.

The Journal of Comparative Neurology
Yoshitaka Hamanaka, Ian A Meinertzhagen

Abstract

The location of proteins that contribute to synaptic function has been widely studied in vertebrate synapses, far more than at model synapses of the genetically manipulable fruit fly, Drosophila melanogaster. Drosophila photoreceptor terminals have been extensively exploited to characterize the actions of synaptic genes, and their distinct and repetitive synaptic ultrastructure is anatomically well suited for such studies. Synaptic release sites include a bipartite T-bar ribbon, comprising a platform surmounting a pedestal. So far, little is known about the composition and precise location of proteins at either the T-bar ribbon or its associated synaptic organelles, knowledge of which is required to understand many details of synaptic function. We studied the localization of candidate proteins to pre- or postsynaptic organelles, by using immuno-electron microscopy with the pre-embedding method, after first validating immunolabeling by confocal microscopy. We used monoclonal antibodies against Bruchpilot, epidermal growth factor receptor pathway substrate clone 15 (EPS-15), and cysteine string protein (CSP), all raised against a fly head homogenate, as well as sea urchin kinesin (antibody SUK4) and Discs large (DLG). All these a...Continue Reading

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Citations

Aug 14, 2012·The Journal of Comparative Neurology·Shamprasad Varija RaghuAlexander Borst
May 2, 2014·The Journal of Comparative Neurology·An-Lun ChinAnn-Shyn Chiang
Sep 3, 2010·Journal of Neurogenetics·Riddhi Majumder, K S Krishnan
Jan 13, 2012·The Journal of Comparative Neurology·Nancy J ButcherIan A Meinertzhagen
Oct 29, 2017·Journal of Neurogenetics·Haritz Plazaola-SasietaMarta Morey
Feb 1, 2011·The Journal of Comparative Neurology·Kazunori ShinomiyaKei Ito
Feb 15, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Yoshitaka HamanakaKentaro Arikawa
Aug 10, 2011·The Journal of Comparative Neurology·Gerd LeitingerDagmar Kolb
Jan 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alicia MansillaMaría José Sánchez-Barrena
Nov 6, 2018·Frontiers in Neuroanatomy·Tina Ghelani, Stephan J Sigrist
Jan 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chong LiMustafa Tekin
Dec 2, 2010·The Journal of Comparative Neurology·Shamprasad Varija RaghuAlexander Borst
Jun 23, 2016·Journal of Neurogenetics·Ian A Meinertzhagen
Feb 12, 2020·Progress in Neurobiology·Cameron B Gundersen
Sep 21, 2021·PLoS Genetics·Samantha YouF Rob Jackson
Jan 30, 2010·Progress in Neurobiology·Tara N Edwards, Ian A Meinertzhagen

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