PMID: 6981649Jul 1, 1982Paper

Immunocytochemical localization of the vitamin D-dependent calcium binding protein in chick duodenum

The Journal of Cell Biology
B ThorensL Orci

Abstract

The vitamin D-dependent calcium binding protein (CaBP) of chick duodenum has been localized by immunocytochemistry and by radioimmunoassay. Light microscopically, CaBP was seen to be present in the absorptive cells of the villi while in other cell types of the villi and the crypts, including goblet cells and endocrine cells, no CaBP was seen. At the electron microscopic level, CaBP was shown to be localized in the cytosol and the euchromatin of the nucleus but not in membrane-bounded cytoplasmic compartments. Quantitative evaluation of the immunocytochemical protein A-gold label showed that the terminal web and the cytosol of basal cellular regions were most highly labeled while the brush border was weakly labeled. The radioimmunoassay evaluation of intestinal subcellular fractions indicated that 96% of the homogenate CaBP is in the cytosol high-speed supernatant fraction. Collectively, these results support the hypothesis that the vitamin D-dependent intestinal CaBP may play a role in either regulation of intracellular calcium concentration or movement of calcium across the brush border membrane from the gut lumen.

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Citations

Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J Davies
Jun 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A BerdalA Nanci
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Apr 9, 2005·Experimental Neurology·Vincenzo Cimini
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Jan 1, 1985·CRC Critical Reviews in Toxicology·P H Bach, J W Bridges
Jan 1, 1985·Critical Reviews in Toxicology·P H Bach, J W Bridges

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