Immunocytochemical localization of the [3H]estradiol-binding protein in rat pancreatic acinar cells

Endocrinology
A GrossmanA R Beaudoin

Abstract

Significant amounts of an estradiol-binding protein (EBP) are present in pancreatic acinar cells. This protein differs from the one found in female reproductive tissues and secondary sex organs (which is commonly referred to as estrogen receptor). EBP has now been purified from rat pancreas and was used as an antigen to induce polyclonal antibodies in rabbits. The antiserum obtained was purified initially by ammonium sulfate fractionation and then still further by interaction with a protein fraction from pancreas that was devoid of estradiol-binding activity. The latter procedure was used to precipitate nonspecific immunoglobulin Gs. Western blot analysis demonstrated that the anti-EBP antibody reacted specifically with a doublet of protein bands having mol wt of 64K and 66K. When this purified antibody was used as an immunocytochemical probe in conjunction with protein-A-gold, acinar cells were labeled on the surface of the endoplasmic reticulum, on the plasma membrane, and in mitochondria. This specific labeling pattern was not observed when preimmune serum was used. No labeling was observed over the nucleus, Golgi apparatus, or zymogen granules with purified anti-EBP antibodies. The unexpected distribution of EBP in both the...Continue Reading

Citations

Nov 11, 1991·The Journal of Steroid Biochemistry and Molecular Biology·P SinghJ C Reubi
Feb 14, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Susanne ArnoldCordian Beyer
May 22, 2009·Journal of Neurochemistry·Susanne Arnold, Cordian Beyer
Mar 18, 2008·IUBMB Life·Anna-Maria G Psarra, Constantine E Sekeris
Mar 29, 2014·Journal of Intensive Care Medicine·Mary AbrahamJames Gasperino
Jan 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Jin Q ChenJames D Yager
Aug 30, 2019·Biology of Sex Differences·Megan E Rosa-Caldwell, Nicholas P Greene
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