PMID: 6112040Mar 30, 1981Paper

Immunocytochemical localization of tyrosine hydroxylase in rat adrenal medulla by the peroxidase labeled antibody method: effects of enzyme activation on ultrastructural distribution of the enzyme

Brain Research
J K StephensP K Nakane

Abstract

A monospecific antibody against tyrosine hydroxylase (TH), purified from a transplantable rat pheochromocytoma, was produced in rabbits. Immunohistochemical techniques were employed in order to determine if a relationship exists between the subcellular distribution of TH and the level of activation of the enzyme in the rat adrenal medulla. Tyrosine hydroxylase activity in adrenals removed from non-stressed rats following pentobarbital anesthesia was found to be 12.9 +/- 1.0 nmol DOPA formed x mg protein-1. The use of ether anesthesia (17.9 +/- 2.0 nmol DOPA formed x mg protein-1), and the administration of electroconvulsive shock (ECS) followed by decapitation (35.9 +/- 2.0 nmol DOPA formed x mg protein-1) was associated with an acute activation of adrenal TH. The subcellular distribution of TH within the cytosol of chromaffin cells from animals subjected to anesthesia or ECS, as determined by immunocytochemical techniques, was similar. In all treatment groups chromaffin cells were found which had TH associated with some chromaffin granules. The percentage of chromaffin granules which appeared to contain TH was lower in animals subjected to ECS plus decapitation as compared with anesthetized animals. These observations suggest ...Continue Reading

References

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Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P R VullietN Weiner

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Citations

Oct 12, 1984·Biochimica Et Biophysica Acta·A L Cahill, R L Perlman
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A L Cahill, R L Perlman
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C W OysterN C Brecha
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K L KelnerH B Pollard
Sep 13, 2008·Acta Histochemica Et Cytochemica·Miki MatsumineHiroaki Ohta

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