PMID: 6373783May 1, 1984Paper

Immunocytochemical studies on the localization of plasma and of cellular retinol-binding proteins and of transthyretin (prealbumin) in rat liver and kidney

The Journal of Cell Biology
M KatoD S Goodman

Abstract

The immunocytochemical localization of cellular retinol-binding protein (CRBP), of plasma retinol-binding protein (RBP), and of plasma transthyretin (TTR) was studied in rat liver and kidney. The studies employed normal rats, retinol-deficient rats, and rats fed excess retinol. Antisera were prepared in rabbits against purified rat CRBP, RBP, and TTR. The primary antibodies and goat anti-rabbit IgG were purified by immunosorbent affinity chromatography, using the respective pure antigen coupled to Sepharose as the immunosorbent. This procedure effectively removed cross-reactive and heterophile antibodies, which permitted the specific staining and localization of each antigen by the unlabeled peroxidase-antiperoxidase method. CRBP was found to be localized in two cell types in the liver, the parenchymal cells and the fat-storing cells. Diffuse cytoplasmic staining for CRBP was seen in all the parenchymal cells. Much more intense staining for CRBP was seen in the fat-storing cells. The prominence of the CRBP-positive fat-storing cells changed markedly with vitamin A status. Thus, these cells were most prominent, and appeared most numerous, in liver from rats fed excess retinol. Both RBP and TTR were localized within liver parench...Continue Reading

References

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Citations

Jan 1, 1985·Histochemistry·F Kiesewetter, P Kugler
Dec 1, 1988·Experimental Eye Research·G P LewisS K Fisher
Jan 1, 1986·Neurochemistry International·C D BridgesR A Alvarez
May 1, 1990·The Journal of Nutritional Biochemistry·M E Davila-BloomD S Goodman
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takahiro TajiriTakashi Ishizaki
Jun 8, 2001·Progress in Retinal and Eye Research·J K McBeeD R Pepperberg
Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·P SauvantV Azaïs-Braesco
Feb 1, 1997·The International Journal of Biochemistry & Cell Biology·A NilssonR Blomhoff
Jun 7, 2000·The Biochemical Journal·N Noy
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M KatoD S Goodman
May 27, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Katrien Van BenedenChristiane Van den Branden
Oct 1, 1995·Neuropathology and Applied Neurobiology·S AlbrechtT Pietsch
Feb 26, 1991·European Journal of Biochemistry·G PerozziS Gaetani
Apr 22, 1995·BMJ : British Medical Journal·N J WatsonH R Atta
May 1, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carlotta RossiDiana Bellovino
Jan 29, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Nobuyo Higashi, Haruki Senoo
Jun 12, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Keisuke NagatsumaTomokazu Matsuura
Nov 27, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Gang ZhaoKen-ichi Yamamura
Jul 31, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Keisuke NagatsumaTomokazu Matsuura
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Oct 18, 2015·European Journal of Pediatrics·Bettina SchmiedchenFlorian J Schweigert
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May 15, 1988·International Journal of Cancer. Journal International Du Cancer·P WahlbergR Willén
Mar 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D E Ong, O Amédée-Manesme
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Dec 1, 1987·Journal of Cellular Physiology·U ErikssonP A Peterson

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