Immunocytochemical study of a temperature-sensitive choline acetyltransferase mutant of Drosophila melanogaster

The Journal of Comparative Neurology
K Ikeda, P M Salvaterra

Abstract

Using a monoclonal antibody to choline acetyltransferase (ChAT), we have identified immunoreactive synaptic terminals in the neuropil regions of the cephalic ganglion of Drosophila melanogaster. This study demonstrates the distribution of antibody-labeled structures within the optic lobe, and then investigates the immunoreactivity altered by mutation in two temperature-sensitive ChAT alleles, chats-1 and chats-2. The general structure of the optic lobe was first observed by means of the silver impregnation technique. Then the presence of ChAT immunoreactivity was determined by the application of antibody [1G4] conjugated with HRP to frozen sections, followed by the 3,3'-diamino-benzidine tetratinct layers, which correspond to the three synaptic layers of the laminarneurons, in the medulla. Also, staining appeared in four distinct layers in the lobula. In addition, weaker staining was observed in the lamina, which corresponds to the retinula cell terminals. Somal layers were not stained. In Canton-S (wild-type), the three medullar layers stain distinctly at both 19 degrees C and 30 degrees C. In chats-1 at 19 degrees C, the stain appeared in the same layers as that of Canton-S, but with somewhat lower density. In chats-2 at 19 d...Continue Reading

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Citations

May 20, 1999·Microscopy Research and Technique·K Yasuyama, P M Salvaterra
Jun 1, 1991·Journal of Neurobiology·M G GorczycaC F Wu
Dec 1, 1996·Journal of Neurocytology·H A BaylisN J Lane
Nov 17, 1992·Biochimica Et Biophysica Acta·E D Gundelfinger, N Hess
Mar 17, 2004·Brain Research. Gene Expression Patterns·P M Salvaterra, T Kitamoto
Nov 1, 1991·Journal of Neurochemistry·H SugiharaP M Salvaterra
Mar 29, 2002·The Journal of Comparative Neurology·Kouji YasuyamaFriedrich-Wilhelm Schürmann
Aug 12, 1999·The Journal of Comparative Neurology·K Yasuyama, I A Meinertzhagen
Nov 1, 1990·Neurochemical Research·L A CarbiniP M Salvaterra
Feb 1, 1993·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Toshihiro Kitamoto, Paul M Salvaterra

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