PMID: 2491623Jan 1, 1989Paper

Immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen

Autoimmunity
C AlquierPierre Carayon

Abstract

We studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. Both open follicles and cells were incubated first with antibodies at +4 degrees C, then with colloidal gold labelled protein A. The topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling was observed at the apical cell surface of the follicles. Furthermore, labelling was restricted to the microvilli level; while smooth membrane territories were devoid of binding sites. In some cases, incubations at 4 degrees C were followed by warming the follicles and cells up to 37 degrees C for 20 minutes in order to study internalization of ligands. Ligands were then observed in intracellular organelles: endosomes and lysosomes. Essentially the same results were observed when human antibodies to the microsomal antigen were used. Controls with microsomal antibodies depleted in anti-peroxidase were negative. In conclusion these findings show that: 1) thyroid peroxidase is present in limited areas on the apical cell surface, 2) labellin...Continue Reading

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Citations

Mar 26, 2002·Molecular and Cellular Endocrinology·Xiaoqing ZhangPeter Arvan
Aug 16, 2005·Archives of Biochemistry and Biophysics·Jean Ruf, Pierre Carayon
Aug 5, 2000·The Journal of Biological Chemistry·X Zhang, P Arvan
May 27, 2005·The Journal of Biological Chemistry·Regina KuliawatPeter Arvan
Jan 1, 1993·Journal of Endocrinological Investigation·C GianiA Pinchera

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