Immunocytochemistry and in situ hybridization studies of pepsinogen C-producing cells in developing rat fundic glands

Cell and Tissue Research
Y B GeFusayoshi Murata

Abstract

The ontogeny of pepsinogen C-producing cells in rat fundic glands was studied by means of light and electron microscopy using an antiserum raised against a synthetic peptide based on rat pepsinogen C. To confirm the immunocytochemistry results, the expression of rat pepsinogen C messenger RNA (mRNA) in the fundic gland was also examined by in situ hybridization using a digoxigenin-labeled RNA probe. In adult rats, pepsinogen C was produced by chief cells, mucous neck cells, and intermediate mucopeptic cells. Pepsinogen C-producing cells appeared in embryos as early as 18.5 days' gestation. The development of these cells could be classified into four stages: (1) 18.5 days' gestation to 0.5 days after birth; (2) 0.5 days to 2 weeks after birth; (3) 3-4 weeks after birth; (4) 4-8 weeks after birth. In embryos and young animals, pepsinogen C-producing cells were mucopeptic cells. By 4 weeks after birth, mucous neck cells could be distinguished morphologically. The maturation stages of the chief cells could be traced by electron microscopy along the longitudinal axis of the rat fundic gland by double-staining with anti-pepsinogen C antibody and periodic acid-thiocarbohydrazide-silver proteinate. Positive reactions for pepsinogen C a...Continue Reading

Citations

Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Elodie NiepceronMarie-Claire Biol-N'garagba
Dec 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lixin ZhuJohn G Forte
May 16, 2002·American Journal of Veterinary Research·Jörg M SteinerDavid A Williams
Dec 22, 2015·Acta histochemica·Ahmed E NoreldinYoshinao Z Hosaka
Sep 13, 2011·Experimental Cell Research·James R GoldenringJason C Mills
Feb 17, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·I GrittiG S Roi
Apr 1, 2017·Scientific Reports·Juliana Guimarães ZulianPatricia Gama
Jul 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Masako SuzukiToshihiko Iwanaga

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