Immunocytochemistry of cytology specimens for predictive biomarkers in lung cancer

Translational Lung Cancer Research
Sinchita Roy-Chowdhuri

Abstract

With a growing number of predictive biomarkers that have emerged in non-small cell lung carcinoma (NSCLC), there has been a paradigm shift in the management of these patients. Of the various predictive biomarker testing methods, immunohistochemistry (IHC) is the most widely available, cost-effective, and commonly used methods. However, most predictive IHC assays are validated primarily on formalin-fixed paraffin-embedded (FFPE) histologic tissue samples and translating these assays to cytologic specimens requires additional and rigorous validation. This is part due to the lack of standardized processing protocols in cytology resulting in a variety of preanalytic variables that can impact the antigenicity of antibodies used for predictive biomarker testing. The review article discusses the various preanalytical and analytical factors that impact immunocytochemistry (ICC) in cytologic specimens and summarizes the current published literature on ALK, ROS1, PD-L1, and other predictive biomarker ICC in cytology.

Citations

Jan 4, 2021·Virchows Archiv : an International Journal of Pathology·Sule Canberk, Marianne Engels
May 7, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Antonino IaccarinoElena Vigliar
May 26, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Yonca KanberManon Auger
May 25, 2021·Frontiers in Medicine·Eva TejerinaMara D Lozano
Oct 11, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Savitri Krishnamurthy, Kechen Ban
Oct 31, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Anthony Maddox, Louise M Smart

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