PMID: 109497Apr 1, 1979

Immunocytochemistry with osmium-fixed tissue. I. Light microscopic localization of growth hormone and prolactin with the unlabeled antibody-enzyme method

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
D G BaskinJ A Parsons

Abstract

Growth hormone and prolactin were localized on thin plastic sections of rat anterior pituitary gland and mammosomatotropic tumor MtTW15 that were fixed with osmium tetroxide (alone,mixed with aldehydes, or after aldehydes). Intense immunocytochemical staining for both antigens was obtained after plastic was removed from sections with an alcoholic solution of sodium hydroxide. The results indicated that antigenic determinants of rat prolactin and growth hormone were not completely destroyed or inactivated by fixation with osmium and embedment in epoxy resin, and that removal of the polymerized epoxy resin was necessary to obtain light microscopic postembedding immunocytochemical staining of these antigens. The results also demonstrated that tissues which have been conventionally processed for morphological evaluation by electron microscopy may be suitable for postembedding immunocytochemical staining of some antigens for light microscopy.

Citations

Jan 1, 1982·Histochemistry·D G Baskin, W L Stahl
Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·H HöflerP U Heitz
Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·H IshikawaT Suzuki
May 1, 1980·The Histochemical Journal·P PetruszJ C Finley
Jan 1, 1981·Journal of Immunological Methods·A G Farr, P K Nakane
Jun 19, 1992·Brain Research. Developmental Brain Research·A KondoH Nagara
Mar 9, 2004·Micron : the International Research and Review Journal for Microscopy·D D'AlessandroF Bianchi
Jan 1, 1988·Acta histochemica·T NinomiyaY Imanishi
Apr 1, 1992·The Journal of Cell Biology·G J SnipesE M Shooter
Jan 1, 1980·Virchows Archiv. A, Pathological Anatomy and Histology·P U Heitz, W Wegmann
Jul 1, 1986·Neuropathology and Applied Neurobiology·G R Moore, C S Raine
Feb 1, 1980·The American Journal of Anatomy·C B RodningI D Wilson
Dec 24, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N SakaguchiB A Schulte
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R Y OsamuraA Matsuno
Jun 1, 1989·The American Journal of Anatomy·A B Johnson, A Bettica
May 20, 1999·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S K KruegerR K Johnson

Related Concepts

Sekumatic
Cytochemistry
Peroxidase-Antiperoxidase Complex Technique
Neoplasms, Experimental
Osmium
Pituitary Gland, Anterior
Pituitary Carcinoma
Prolactin
Recombinant Growth Hormone

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.