Immunocytological and preliminary immunohistochemical studies of prothymosin alpha, a human cancer-associated polypeptide, with a well-characterized polyclonal antibody.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Persefoni KlimentzouEvangelia Livaniou

Abstract

Prothymosin alpha (ProTalpha) is a nuclear polypeptide of great biological and, possibly clinical, importance, because its expression levels have been associated with early diagnosis/prognosis of human cancer. It is therefore interesting to raise easily available and cost-effective antibodies that would be applied to develop reliable ProTalpha immunodiagnostics. In this study, New Zealand white rabbits and laying hens were parallel immunized against intact ProTalpha or the synthetic fragments ProTalpha[1-28], ProTalpha[87-109], and ProTalpha[101-109], all conjugated to keyhole limpet hemocyanin (KLH). The corresponding antibodies G and Y were immunochemically evaluated in parallel with ELISA and Western blot systems and applied to fluorescence immunocytology experiments using various cancer cell lines and normal cells. The antibody G raised against ProTalpha[101-109]/KLH had excellent functional characteristics in the Western blot and immunocytology experiments, where the fluorescent signal was almost exclusively shown in the cell nucleus independently of the cells assayed. The above antibody has been applied to preliminary IHC staining of human cancer prostate tissues, leading to a high percentage of clearly and intensively st...Continue Reading

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Citations

Apr 16, 2014·Virchows Archiv : an International Journal of Pathology·Yuh-Shyan TsaiTzong-Shin Tzai
Dec 2, 2009·Applied Biochemistry and Biotechnology·Ilias PapasarantosEvangelia Livaniou
Mar 1, 2012·Cancer Immunology, Immunotherapy : CII·Kyriaki IoannouOurania E Tsitsilonis
Jan 1, 2009·Folia Histochemica Et Cytobiologica·Marek Pawlikowski, Katarzyna Winczyk
Jan 15, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Chrysoula-Evangelia KarachaliouEvangelia Livaniou
Aug 8, 2019·Current Medicinal Chemistry·Chrysoula-Evangelia KarachaliouEvangelia Livaniou

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Methods Mentioned

BETA
ELISA
FCS
Fluorescence
confocal microscopy

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