Immunocytological detection of AAF-DNA adducts in HeLa cell nuclei

Cancer Letters
E SageR Bases

Abstract

Acetylaminofluorene-DNA adducts (AAF-DNA) were detected in the nuclei of HeLa cells exposed to N-acetoxy-2-acetylaminofluorene (N-Ac-AAF), using an immunocytological technique and specific antibodies directed against AAF modified DNA. The proportion of cells exhibiting specific nuclear immunoreactivity was dose-dependent. The time course of disappearance of adduct specific nuclear immunoreactivity was compared with removal of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) and other adducts.

References

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Citations

Nov 1, 1990·Mutation Research·L den EngelseE Scherer
Feb 1, 1983·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J J PestkaF S Chu
Mar 1, 1985·Biochimie·M Leng

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