Immunocytological urinalysis and monocyte chemotactic peptide-1 in renal transplant recipients with polyomavirus replication

Transplantation Proceedings
M BoratyńskaM Klinger

Abstract

In some patients polyomavirus replication induces chronic tubulointerstitial inflammation in the transplanted kidney. The aim of this study was to investigate whether immunocytological urinalysis and monocyte chemoattractant protein-1 (MCP-1) assays could be used for an early diagnosis of nephropathy for patients with polyomavirus replication. We analyzed 1189 urine sediments from 174 renal allograft recipients who were transplanted between 2000 and 2005. Decoy cells were identified by an immunofluorescence method using specific antibodies (JC/BK monoclonal antibody). A similar method was used to detect CD3(+), CD14(+), and HLA-DR(+) cells with appropriate antibodies. The urinary excretion of MCP-1 was assayed by enzyme-linked immunosorbent assay. The results of urine sediment analysis and MCP-1 concentrations were compared with those of patients with stable graft function (control group n = 65). In 17 patients (10%) decoy cells were identified in urine. In 12 patients polyomavirus DNA was detected in plasma or urine by a polymerase chain reaction method. Polyomavirus nephropathy was diagnosed in eight patients by the presence of intranuclear viral inclusions or immunohistochemical staining with SV40 large T-antigen specimens f...Continue Reading

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Citations

Mar 20, 2008·Clinical Journal of the American Society of Nephrology : CJASN·Aaron Dall, Sundaram Hariharan
Jan 12, 2016·Viral Immunology·Ashraf KariminikShahriar Dabiri
Jul 7, 2012·Clinical Transplantation·Kazuaki YamanakaYasuji Ichikawa
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Jun 5, 2021·Virology Journal·Mohammad Hassan Mohammadi, Ashraf Kariminik

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