Immunocytology shows the presence of tobacco etch virus P3 protein in nuclear inclusions

Journal of Structural Biology
W G Langenberg, L Zhang

Abstract

Intracellular localization studies of various potyvirus proteins have been made in hope of finding clues to their function(s). Immunocytological studies localized many of the tobacco etch virus (TEV)-encoded proteins in infected cells. We used antiserum against the nonstructural P3 protein of TEV to determine the subcellular location of the P3 protein in ultrathin sections of virus-infected cells. Immunogold labeling with the antiserum showed labels associated with nucleoli, nuclei, or NIs, Absorption of antiserum with purified NIs or P3 protein resulted in no labeling. TEV NIs are known to contain a bifunctional genome-linked protein-viral proteinase (NIa-VPg) and RNA-dependent RNA polymerase (NIb). It appeared that the TEV P3 protein was a third nonstructural viral protein of NIs of TEV if the NIa-VPg is considered one protein. The presence of P3 in NIs was also supported by Western blot assays. P3 protein in the nucleolus and nucleus could indicate that it, too, is involved in early stages of viral replication.

References

Jan 1, 1992·The Journal of General Virology·J L RiechmannJ A García
Dec 1, 1991·Virology·E Rodríguez-Cerezo, J G Shaw
Apr 1, 1989·The Journal of General Virology·C RobagliaF Casse-Delbart
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1993·The Journal of General Virology·E Rodríguez-CerezoJ G Shaw

❮ Previous
Next ❯

Citations

Jun 15, 2007·Virus Genes·Sarasate EiamtanasateYun-Kiam Yap
Feb 28, 2001·Virus Research·S Urcuqui-InchimaF Bernardi
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Carol E JennerJohn A Walsh
Jul 27, 2007·Journal of Virology·Stephanie A Montgomery, Robert E Johnston
Dec 4, 2012·Viruses·Jiri SochorRene Kizek
Jan 15, 2014·Journal of Experimental Botany·Andrey G Solovyev, Eugene I Savenkov
Mar 14, 2012·Micron : the International Research and Review Journal for Microscopy·Katarzyna Otulak, Grażyna Garbaczewska
Dec 10, 2015·Molecular Plant-microbe Interactions : MPMI·Flora SánchezFernando Ponz
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Gadi PelegShmuel Wolf
Apr 12, 2014·The Journal of General Virology·K I IvanovK Mäkinen
Oct 19, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·K C Lee, S M Wong
Jul 18, 2003·The Journal of General Virology·Michael E Taliansky, David J Robinson
Jul 24, 2004·Biochemical Society Transactions·S H KimM Taliansky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.