Immunodetection of benzo[a]pyrene adducts in ovarian cells of women exposed to cigarette smoke

Molecular Human Reproduction
M T ZenzesR Bielecki

Abstract

Benzo[a]-pyrene (B[a]P) is a potent mutagen and carcinogen present in cigarettes. We report here on immunodetection and quantification of B[a]P-DNA adducts in granulosa-lutein cells of patients undergoing in-vitro fertilization (IVF) and embryo transfer, who were exposed to cigarette smoke. Follicular fluids (FF) and granulosa-lutein cells were obtained from the same follicular aspirate from 32 women self-reported as active smokers, passive smokers, or non-smokers. Cells were immunostained with 5D11, an anti-B[a]P diolepoxide monoclonal antibody that recognizes DNA adducts. Cotinine, a reliable marker for recent smoke exposure and dose, was assessed by radioimmunoassay in 32 FF samples. Individual scores of cell immunoreactivity were highly correlated with FF cotinine concentrations. Evaluations of immunostaining intensity in 9770 granulosa-lutein cells from the 32 women revealed higher average scores in active and passive smokers, relative to non-smokers. In passive smokers the average level of cell immunostaining was 63% of that of active smokers. These relationships provide quantitative evidence that B[a]P-DNA adduct levels are related to smoke exposure and dose, both recent and long term. Immunostaining was confined to the ...Continue Reading

Citations

Feb 23, 2013·Journal of Assisted Reproduction and Genetics·Yves MénézoKay Elder
Jan 30, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Daniel J Wilks, Alastair W M Hay
May 9, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P D TerryT E Rohan
Jul 29, 2010·Zygote : the Biology of Gametes and Early Embryos·Yves MénézoMarc Cohen
Nov 24, 2011·Chemical Research in Toxicology·Kun-Ming ChenKaram El-Bayoumy
Oct 23, 2010·Journal of Toxicology and Environmental Health. Part a·Aramandla RameshMohammad S Niaz
Aug 6, 2010·Human Reproduction Update·C DechanetH Dechaud
Jan 13, 2010·Menopause : the Journal of the North American Menopause Society·Beth J PlanteAnne Z Steiner
Jun 6, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Nora L NockBenjamin A Rybicki
Feb 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Benjamin A RybickiDeliang Tang
Mar 1, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·J CorralesK L Willett
Mar 28, 2008·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Warren G FosterMiguel M Dominguez
Mar 5, 2013·Cancer Causes & Control : CCC·Mette T FaberUNKNOWN Ovarian Cancer Association Consortium
Sep 5, 2008·Expert Review of Vaccines·Thomas M Luby
Nov 28, 2012·Gynécologie, obstétrique & fertilité·Y MénézoM Brack
Sep 21, 2011·Gynécologie, obstétrique & fertilité·C DechanetH Dechaud
Apr 5, 2011·Microbes and Infection·R V Schoborg
Dec 1, 2010·Reproductive Biomedicine Online·Avi Ben-HaroushJacob Farhi
Mar 18, 2005·The Journal of Obstetrics and Gynaecology Research·Yoshimitsu NiwaUNKNOWN JACC Study Group
Oct 6, 2007·International Journal of Cancer. Journal International Du Cancer·Inger T GramElisabete Weiderpass
Jun 17, 2011·International Journal of Cancer. Journal International Du Cancer·Inger T GramRudolf Kaaks
Apr 6, 2005·Reproductive Toxicology·Ildikó SinkóIstván Raskó
Jan 22, 2005·Gynecologic Oncology·Allison W KurianAlice S Whittemore
Sep 29, 2006·Gynecologic Oncology·Susan J JordanPenelope M Webb
May 24, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiefan FangKristine L Willett
Nov 26, 2010·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Adriana Fortunato, Elisabetta Tosti
Mar 29, 2002·Environmental and Molecular Mutagenesis·Andrew RundleFrederica Perera
Dec 14, 2016·British Journal of Cancer·Idlir LicajInger Torhild Gram
Dec 17, 2004·Human Reproduction Update·Edward V YounglaiWarren G Foster
Jul 3, 2002·Epidemiology·Francesmary ModugnoCarrie M Cottreau
May 25, 2013·Human & Experimental Toxicology·P V RekhadeviA Ramesh
Jul 13, 2012·Zygote : the Biology of Gametes and Early Embryos·Yves MénézoBrian Dale
Dec 13, 2016·Journal of Applied Genetics·Błaszczyk Ewa, Mielżyńska-Švach Danuta
Dec 18, 2004·Cancer Research·Benjamin A RybickiDeliang Tang
Jun 14, 2020·Reproductive Biomedicine Online·Antoine NetterJeanne Perrin

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