Immunodetection of telomerase-like immunoreactivity in normal and regenerating tail of amphibians suggests it is related to their regenerative capacity

Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology
Lorenzo Alibardi

Abstract

The high regenerative power of amphibians suggests that numerous signaling pathways remain active in larvae and adult tissues, including those stimulated by telomerases. The protein component of Telomerase (TERT) shows high amino acid homology in numerous vertebrates and we have utilized an antibody made for a lizard telomerase to detect a putative telomerase in amphibians tissues based on 60-75% epitope identity. Western blotting studies indicate that telomerase is constitutionally present in normal and regenerating tails of tadpoles of the anuran Rana dalmatina. In the neotenic urodele Ambystoma mexicanum the enzyme is detectable in the normal tail and increases during tail regeneration. Immunofluorescence shows that telomerase is localized in both epidermal and mesenchymal cells of normal and regenerating tail of A. mexicanum, especially in their nuclei. The ultrastructural localization with gold or gold-silver intensified confirms that telomerase is mainly present in the nucleus, in particular in discrete regions of the nucleoplasm identified as Cajal bodies and in the nucleolus, sites known in mammalian cells to be involved in the processing of the enzyme. Telomerases are present in normal and even more in regenerating tis...Continue Reading

References

Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C ScalaG C Manara
Jan 1, 1991·Glia·P Ferretti, J P Brockes
Jan 1, 1984·Differentiation; Research in Biological Diversity·D L Stocum
Apr 28, 2001·Neoplasia : an International Journal for Oncology Research·E HiyamaJ W Shay
Jul 23, 2002·Molecular Biology of the Cell·Yun Wah LamAngus I Lamond
Jan 28, 2003·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Sylvia BousmanJanis Shampay
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mark HartyAnthony L Mescher
Jun 6, 2003·Cell·Elly M Tanaka
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Mario Cioce, Angus I Lamond
Mar 6, 2007·Development, Growth & Differentiation·Makoto MochiiIsshin Shikata
Oct 2, 2007·BMC Evolutionary Biology·Eric VilliardStéphane Roy
Nov 16, 2007·Alternatives to Laboratory Animals : ATLA·Laurens N RubenMichael Balls
May 16, 2008·Experimental Biology and Medicine·Lynne W ElmoreShawn E Holt
Jun 9, 2010·TheScientificWorldJournal·Kenyon S Tweedell
Jul 2, 2010·Journal of Cell Science·Zhu-Hong LiMichael P Terns
Feb 4, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·David L Stocum, Jo Ann Cameron
Feb 25, 2011·PloS One·Monique AnchelinMaría L Cayuela
Nov 9, 2012·Journal of Cell Science·Agnel Sfeir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.