Immunodetection of the large form of the gamma 2 subunit of mammalian GABAA receptor

Brain Research
I San JuanP Calvo

Abstract

Two forms of the GABAA receptor gamma 2-subunit, named short (gamma 2S) and long (gamma 2L), and generated by alternative RNA splicing, have been identified in mammalian brain by molecular cloning techniques. We have produced antibodies against a synthetic peptide containing the 8-amino acid insertion present in the long form but not in the short one. Using the antipeptide serum, we have identified the gamma 2L subunit in membrane preparations of GABAA receptors from rat and mouse cerebellum and its cellular location in cerebellum.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M FritschyH Möhler
Nov 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D R Burt, G L Kamatchi
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P WhitingL L Iversen
Oct 30, 1990·European Journal of Pharmacology·D BenkeH Mohler

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