PMID: 9534208Apr 16, 1998Paper

Immunodetection of the murine chemotactic protein CP-10 in bleomycin-induced pulmonary injury

Pathology
R K KumarCarolyn L Geczy

Abstract

The murine S-100 protein designated CP-10 is a potent chemotactic factor for phagocytic cells, exhibiting optimal activity in the picomolar range. We assessed the role of this cytokine in the inflammatory response to pulmonary injury following intratracheal administration of bleomycin to mice. In the lungs of normal animals, strong cytoplasmic immunostaining for CP-10 was demonstrable in all recognisable neutrophil leucocytes sequestered within alveolar capillaries. Following induction of pulmonary inflammation in susceptible C57BL/6 mice, numerous CP-10-positive neutrophils were observed, but many of the recruited neutrophils did not exhibit staining for CP-10. No other cells were immunoreactive. The concentration of CP-10 in bronchoalveolar lavage (BAL) fluids from normal mice and mice administered intratracheal saline was below the level of detection by enzyme immunoassay. In contrast, nanomolar levels of CP-10 were detected in unconcentrated BAL fluids from C57BL/6 mice after bleomycin-induced injury, and the presence of monomeric CP-10 was demonstrable by Western blotting. Elevation of CP-10 levels correlated with the influx of inflammatory cells in C57BL/6 mice, but was not demonstrable in BAL fluids from BALB/c mice, whi...Continue Reading

References

Jun 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R K Kumar
Jan 1, 1983·The American Review of Respiratory Disease·D J SchrierS H Phan
Feb 1, 1994·DNA and Cell Biology·S E IismaaC L Geczy
Dec 3, 1993·Journal of Immunological Methods·A Herráez
Jul 1, 1993·Experimental Lung Research·C Selby, W MacNee
Apr 1, 1996·Infection and Immunity·M KocherL Geczy C
Mar 27, 1996·Biochemical and Biophysical Research Communications·T KomadaT Tanaka
Jul 1, 1996·The Journal of Investigative Dermatology·T JinquanK Thestrup-Pedersen
Oct 11, 1996·Biochimica Et Biophysica Acta·C Geczy

❮ Previous
Next ❯

Citations

Mar 20, 1999·The Journal of Biological Chemistry·C A HarrisonC L Geczy
Jul 11, 2001·The Journal of Biological Chemistry·M J RafteryC L Geczy
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K XuC L Geczy
Jun 27, 2018·Frontiers in Immunology·Siwen WangJian Ma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.