Immunodiagnosis of opisthorchiasis using parasite cathepsin F

Parasitology Research
Salma TeimooriB Sripa

Abstract

Opisthorchis viverrini, a food-borne trematode parasite endemic in the lower Mekong countries, is conventionally diagnosed by stool examination. However, parasitological stool-based diagnosis can be unreliable in light infections. The goal of this study was to develop the immunodiagnosis of opisthorchiasis using cathepsin F cysteine protease of O. viverrini in both indirect and sandwich ELISA assays. A recombinant O. viverrini cathepsin F (rOv-CF) of 40 kDa was expressed in E. coli strain BL21 (DE3), affinity purified, and deployed in ELISA assays. Human sera from 272 cases were investigated by indirect rOv-CF-based ELISA. Positive antibody response to rOv-CF was found in 137 out of 272 cases (50.37 %) using a cutoff OD (0.400) determined by ROC analysis. In comparison to parasitological stool examined for fluke eggs, the gold standard, the rOv-CF indirect ELISA showed a sensitivity and specificity of 62.1 and 84.05 %, respectively. Serum antibody levels correlated well with egg counts per gram feces (EPG) (P < 0.001). In addition, chicken IgY antibody raised against rOv-CF was tested in a sandwich ELISA for detection of coproantigen in the feces of experimentally infected hamsters. The sandwich ELISA using this chicken IgY in ...Continue Reading

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Citations

May 4, 2016·Parasitology International·Salma TeimooriBanchob Sripa
Apr 27, 2017·Journal of Proteomics·Karina M MonteiroHenrique B Ferreira
Aug 9, 2017·Gut and Liver·Piyapan PrueksapanichRungsun Rerknimitr
Sep 5, 2019·The American Journal of Tropical Medicine and Hygiene·Lakkhana SadaowPewpan M Intapan
Apr 5, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Khamphanavanh SaiyachakSupannee Promthet
Apr 22, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Weeraya PhupiewkhamWanchai Maleewong

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