Immunodiagnosis of parasitic zoonoses: sensitivity and specificity of in vitro lymphocyte proliferative responsiveness using nematode antigens purified by affinity chromatography

Transactions of the Royal Society of Tropical Medicine and Hygiene
J S Welch, C Dobson

Abstract

The sensitivity of in vitro lymphocyte proliferative responsiveness using antigens purified by affinity chromatography, was greater than that of three serological tests and allowed the specific identification of Dirofilaria immitis, Toxocara canis, Angiostrongylus cantonensis and Ascaris lumbricoides in animals sensitized with parasite antigens, and the diagnosis of natural (D. immitis, T. canis and Angiostrongylus cantonensis) and zoonotic nematode infections. Lymphocytes remained viable in whole blood suspensions in RPM 1640 in a polystyrene insulation box at air temperature for at least 12 hours and reacted in the cell-mediated immunity test (CMIT) without loss of response. The CMIT proved useful in the early immunodiagnosis of nematode infections and showed that parasite antigens purified by affinity chromatography retained a high degree of specificity and sensitivity in both the CMIT and serological tests.

References

Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·J S Welch, C Dobson
Jun 1, 1979·Australian Veterinary Journal·J S WelchC Freeman
Dec 1, 1976·Nihon juigaku zasshi. The Japanese journal of veterinary science·K YoshimuraH Yoshida
Jan 11, 1975·Advances in Parasitology·J E Larsh, N F Weatherly
Jan 1, 1976·The American Journal of Tropical Medicine and Hygiene·K Yoshimura, E J Soulsby
May 1, 1979·Australian Veterinary Journal·J S WelchC Freeman

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Citations

Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·J S WelchS Chopra
Jan 1, 1981·Journal of Immunological Methods·A Voller, D De Savigny

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