PMID: 109402Jan 1, 1979

Immunodiffusion studies of various structural preparations from mycobacterial cells

International Archives of Allergy and Applied Immunology
R BakerO Ouchterlony

Abstract

Various structures and other preparations from mycobacterial cells were analyzed by immunodiffusion. The preparations were obtained from four strains referred to the species Mycobacterium bovis (BCG), Mycobacterium fortuitum, Mycobacterium phlei and Mycobacterium smegmatis. They represented cell walls (CW), culture filtrates (CF), artificially disintegrated cell material (XP), protoplasms (PP), crude ribosomes (CR), ribosomal 50S subunits (50S), ribosomal 30S subunits (30S), ribosomal 16S core particles (16S) and ribosomal-free fractions (UCS). The preparations were analyzed by reference precipitation systems based on CF, CR and 30S preparations. Serological comparisons were made with material from the same species and cross-testing between material from different species was not performed. It was shown that from the precipitinogenic point of view the CF and XP materials were very similar and that they both contained protoplasmic material to a large extent. Furthermore, it was found that ribosomal precipitinogens constitute an important part of the precipitinogens in PP as well as in the CF and the XP preparations. The ribosomal precipitinogens were found to represent the two subunits as well as the 16S core particle. One ribos...Continue Reading

Citations

Jan 1, 1982·Critical Reviews in Microbiology·S D Chaparas

Related Concepts

Antigens, Bacterial
Cell Wall
Protoplasm
Immunodiffusion Measurement
Genus Mycobacterium
Mycobacterium phlei
Immune Precipitates
Ribosomes

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