Immunodominant antigen of Actinobacillus actinomycetemcomitans Y4 in high-responder patients.

Infection and Immunity
J V CalifanoJ G Tew

Abstract

This study was undertaken to look for characteristics of the immunodominant antigen(s) of Actinobacillus actinomycetemcomitans Y4 that might help explain the high antibody titers in periodontitis patients. Radioimmunoassays (RIA) were performed on sera from 481 patients; sera from the 32 patients with the highest anti-Y4 titers (above 128,000 RIA U/ml) were further analyzed. Y4 antigen was boiled for 45 min or treated with papain, and antibody responses were analyzed by RIA and Western blotting (immunoblotting). In addition, carbohydrate was purified from Y4 and examined by Western blotting. The results indicated that the immunodominant antigen of Y4 in high responders was stable after papain treatment or boiling for 45 min. Papain or boiling eliminated protein bands but a large diffuse band persisted on Western blots. With increasing dilutions of sera, bands on Western blots corresponding to protein antigens disappeared, while the large diffuse band resembling that of carbohydrate persisted. Partially purified Y4 carbohydrate contained the large diffuse band. Double-immunodiffusion analysis indicated that rabbit serotype b-specific antiserum and patient sera recognized the same antigen. When the carbohydrate extract was passed...Continue Reading

References

Nov 1, 1977·Applied and Environmental Microbiology·P B HylemonP V Phibbs
Mar 1, 1986·The Journal of Infectious Diseases·D L Kasper
Sep 1, 1987·Journal of Periodontal Research·W E Moore
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Citations

Jan 1, 1991·Journal of Periodontal Research·J C GunsolleyH A Schenkein
Jun 1, 1994·Periodontology 2000·J L Ebersole, M A Taubman
Oct 1, 1994·Periodontology 2000·H A Schenkein, T E Van Dyke
Mar 1, 1996·Journal of Periodontology·Mark E Wilson, John R Kalmar
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J L Ebersole
Sep 1, 1992·Journal of Periodontal Research·M A TaubmanJ L Ebersole
Nov 1, 1995·Journal of Clinical Periodontology·W P McArthurN I Magnusson
Aug 1, 1996·Oral Microbiology and Immunology·N Takamatsu-MatsushitaT Koga
Aug 1, 1993·Journal of Periodontology·W P McArthur, W B Clark
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M PodmoreD F Kinane
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Feb 1, 1994·Journal of Clinical Periodontology·J L EbersoleM N Sandoval
Oct 1, 1994·Journal of Clinical Periodontology·I MagnussonW B Clark
May 1, 1996·Immunological Investigations·J L EbersoleM J Steffen
May 1, 1994·Journal of Periodontology·Bryan S Michalowicz
Apr 11, 2006·Canadian Journal of Microbiology·Rodrigo Estêvão TeixeiraPaula Prazeres Magalhães
Aug 1, 1993·Journal of Periodontology·William P McArthur, William B Clark

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