PMID: 8602330Aug 1, 1995Paper

Immunodominant antigens of Porphyromonas gingivalis in patients with rapidly progressive periodontitis

Oral Microbiology and Immunology
H A ChenR C Page

Abstract

W studied 4 isolates of Porphyromonas gingivalis, ATCC 33277, 381, A7A1-28, and W50, to identify major cell surface antigens and select the best strain from which to obtain antigen for a test vaccine. Immunoglobulin G (IgG) titers measured by enzyme-linked immunosorbent assay using whole-cell sonicates as antigen were significantly elevated for the sera of 64 rapidly progressive periodontitis patients relative to sera of 30 normal control subjects for each of the 4 strains studied. Western blots were prepared for all 4 strains and developed using sera from 22 patients and 20 control subjects to identify and determine the frequency of antibody-binding components. The intensity of binding by patient sera was greatest for the 75-kDa and 55-kDa components. The 43-kDa component was also widely recognized. Strains ATCC 33277 and 381 appeared to be antigenically similar. Because of the higher serum antibody titers, the larger proportion of seropositive patients and higher frequency of binding to specific protein components in Western blots, our efforts were focussed on strain ATCC 33277. Whole-cell sonicates, proteinase K-digested sonicate, lipopolysaccharide, capsular polysaccharide, and whole-cell protein fractions were prepared and...Continue Reading

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Citations

Mar 12, 2008·Infection and Immunity·Mehmet A EskanDenis F Kinane
Dec 5, 2003·Journal of Periodontology·Satoru InagakiKatsuji Okuda
Sep 1, 2005·Journal of Clinical Periodontology·G Rutger Persson
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M PodmoreD F Kinane
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
Jan 12, 2001·Oral Microbiology and Immunology·S I FarquharsonG R Gray
Aug 1, 1996·Oral Microbiology and Immunology·D VaselR C Page
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R M Love, H F Jenkinson
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E GemmellG J Seymour
Jan 12, 2001·Oral Microbiology and Immunology·Q FanR C Page

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