Immunodominant domains present on the Bordetella pertussis vaccine component filamentous hemagglutinin

The Journal of Infectious Diseases
E LeiningerM J Brennan

Abstract

To identify immunologically important domains on filamentous hemagglutinin (FHA), a Bordetella pertussis protein included in new acellular pertussis vaccines (ACPVs), a series of monoclonal antibodies, sera from infants vaccinated with ACPVs or whole cell pertussis vaccine (WCPV), and sera from patients with pertussis were analyzed by immunoblots containing FHA fragments and recombinant FHA proteins. Immunodominant domains located at the COOH-terminus of FHA (type I domain) and near the NH2-terminus (type II domain) were defined by the reactivity with monoclonal antibodies. The sera from patients with pertussis and sera from infants vaccinated with WCPV or with 6 different investigational ACPVs specifically recognized well-defined regions within the type I and type II domains. Identification of these prominent immunologic epitopes on FHA should be useful for the construction of more well-defined pertussis vaccines and for the interpretation of human serologic responses, which may correlate with efficacy of pertussis vaccines.

Citations

Mar 12, 2003·The Journal of Experimental Medicine·Loic CoutteFrancoise Jacob-Dubuisson
Oct 30, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hossein Asgarian-OmranFazel Shokri
Apr 12, 2016·Journal of Microbiological Methods·Song F LeeScott A Halperin
Nov 25, 2003·Scandinavian Journal of Immunology·O Poulain-GodefroyG Riveau
Apr 25, 2002·Molecular Microbiology·Franco D MenozziCamille Locht
Sep 27, 2006·Molecular Microbiology·Joseph Mazar, Peggy A Cotter
Jul 29, 2000·Current Problems in Pediatrics·M R Schleiss, K Dahl

❮ Previous
Next ❯

Related Concepts