PMID: 7536108May 1, 1995Paper

Immunodominant epitope regions of HIV-1 reverse transcriptase: correlations with HIV-1+ serum IgG inhibitory to polymerase activity and with disease progression

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
B Grimison, J Laurence

Abstract

Anti-HIV-1 polymerase antibodies are present in high titer throughout the course of HIV disease. The possibility that a subset of these immunoglobulins might represent an adaptive immune response was suggested by several studies, from this laboratory and others, identifying IgGs capable of inhibiting the catalytic activity of reverse transcriptase (RT) in asymptomatic HIV+ individuals, which were absent from most AIDS patients. We have now determined the dominant B-cell epitope segments of RT recognized during natural HIV-1 infection, with one epitope region, amino acids 244-269, recognized by the majority of HIV-1 positive sera. We have also correlated IgG RT inhibitor activity with amino acids 157-178, corresponding to sequences previously identified by monoclonal antibody mapping and site-directed mutagenesis as necessary for the maintenance of polymerase function. Absence of reactivity to synthetic peptides prepared from this latter region correlated with both advanced HIV disease in a cross-sectional survey of 57 HIV-1+ individuals, and progression of clinical symptoms in a small cohort followed longitudinally. Given current interest in examining diverse components of HIV in development of peptide-based protective vaccines...Continue Reading

Citations

Aug 10, 2000·Nature Biotechnology·P De BerardinisJ Guardiola
Oct 12, 2007·Biochemical and Biophysical Research Communications·Iris Oz GleenbergAmnon Hizi

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