PMID: 9423849Jan 10, 1998Paper

Immunodominant major outer membrane proteins of Ehrlichia chaffeensis are encoded by a polymorphic multigene family

Infection and Immunity
N OhashiY Rikihisa

Abstract

Several immunodominant major proteins ranging from 23 to 30 kDa were identified in the outer membrane fractions of Ehrlichia chaffeensis and Ehrlichia canis. The N-terminal amino acid sequence of a 28-kDa protein of E. chaffeensis (one of the major proteins) was determined. The gene (p28), almost full length, encoding the 28-kDa protein was cloned by PCR with primers designed based on the N-terminal sequence of the E. chaffeensis 28-kDa protein and the consensus sequence between the C termini of the Cowdria ruminantium MAP-1 and Anaplasma marginale MSP-4 proteins. The p28 gene was overexpressed, and antibody to the recombinant protein was raised in a rabbit. The antibody and serum from a patient infected with E. chaffeensis reacted with the recombinant protein, three proteins (29, 28, and 25 kDa) of E. chaffeensis, and a 30-kDa protein of E. canis. Immunoelectron microscopy with the rabbit antibody revealed that the antigenic epitope of the 28-kDa protein was exposed on the surface of E. chaffeensis. Southern blot analysis with a 32P-labeled p28 gene probe revealed multiple copies of genes homologous to p28 in the E. chaffeensis genome. Six copies of the p28 gene were cloned and sequenced from the genomic DNA by using the same ...Continue Reading

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Citations

Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·F R RurangirwaG H Palmer
Oct 18, 2000·Infection and Immunity·A F BarbetG H Palmer
Feb 24, 2001·Annals of the New York Academy of Sciences·K M KocanA F Barbet
Jul 16, 2003·Annals of the New York Academy of Sciences·Patricia A Crocquet-ValdesDavid H Walker
Jul 23, 2003·Infection and Immunity·Julia Shu-Yi Li, Gary M Winslow
Feb 17, 2006·PLoS Genetics·Julie C Dunning HotoppHervé Tettelin
Sep 22, 2006·Cellular Microbiology·Jian-zhi ZhangXue-jie Yu
Dec 19, 2006·Journal of Bacteriology·Haibin HuangYasuko Rikihisa
Jun 22, 2010·Journal of Bacteriology·Yumi KumagaiYasuko Rikihisa
Oct 19, 2013·Journal of Veterinary Science·Rosiane Nascimento AlvesMarcelo EmÍlio Beletti
Jan 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nicola E CollinsBasil A Allsopp
Jan 15, 2003·Clinical Microbiology Reviews·Christopher D Paddock, James E Childs
Aug 8, 2007·Infection and Immunity·Kimberly A NetheryJere W McBride
Sep 9, 2010·Expert Review of Vaccines·Jere W McBride, David H Walker
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Sep 23, 2003·Infection and Immunity·Quan LinNing Zhi
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Jul 16, 2003·Annals of the New York Academy of Sciences·Gary M WinslowJulia Shu-Yi Li
Sep 13, 2014·Environmental Microbiology·Karin AistleitnerMatthias Horn
Oct 22, 2015·Annual Review of Microbiology·Yasuko Rikihisa
Nov 18, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Melinda J WilkersonRoman R Ganta

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