Immunoelectrochemical assays for bacteria: use of epifluorescence microscopy and rapid-scan electrochemical techniques in development of an assay for Salmonella

Analytical Chemistry
J D BrewsterC G Crawford

Abstract

Immunoelectrochemical sensors in which the sensor surface functions as both analyte capture phase and electrochemical detector have recently been developed for bacteria analysis. The speed and sensitivity of these devices make them very attractive for applications such as the detection of pathogenic microorganisms in food and water. However, the development and optimization of assays utilizing these sensors can be complicated by undesired interactions between the capture and detection functions. Modification of the sensor to achieve improvements in one function can have deleterious effects on the other function, and such effects can be difficult to diagnose and correct. In the course of investigations on immunoelectrochemical detection of Salmonella, we developed a rapid, nondestructive epifluorescence microscopy method to determine bacteria capture efficiency. This method enabled us to study capture and detection functions independently and efficiently identify performance-limiting factors. Rapid-scan electrochemical methods were used to optimize detection sensitivity and to provide diagnostic information on detection performance.

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Citations

Aug 22, 2007·Analytical and Bioanalytical Chemistry·Jie XuDavid S Gottfried
Feb 20, 2008·Analytical and Bioanalytical Chemistry·Ilaria Palchetti, Marco Mascini
Jun 9, 1998·Journal of Immunological Methods·J D Brewster, R S Mazenko
Jun 24, 1999·Journal of Microbiological Methods·R S MazenkoJ D Brewster
Apr 23, 2003·Biosensors & Bioelectronics·Namrata SippyDavid C Cowell
Sep 13, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Vepa K RaoKrishnamurthi Sekhar
Apr 5, 2008·Analytical and Bioanalytical Chemistry·Andrew G GehringJeffrey D Brewster
Apr 15, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Zhouping WangGuowei Le
Mar 3, 2005·Biosensors & Bioelectronics·Pierre N FlorianoJohn T McDevitt
Jun 6, 2015·European Biophysics Journal : EBJ·Minsuk KongSangryeol Ryu
Feb 28, 2006·Nanotechnology·Leidong Mao, Hur Koser
Dec 17, 2008·Analytical Chemistry·Arghavan ShabaniRosemonde Mandeville
May 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Cornel Wolf, Qin Li
Aug 18, 2018·Analytical Chemistry·Kengo IshikiTsutomu Nagaoka
Jan 15, 2005·Analytical Chemistry·Svetlana FarrellWilliam R Heineman

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