Immunoelectron microscopy in embryos

Methods : a Companion to Methods in Enzymology
W D Sierralta

Abstract

Immunogold labeling of proteins in sections of embryos embedded in acrylate media provides an important analytical tool when the resolving power of the electron microscope is required to define sites of protein function. The protocol presented here was established to analyze the role and dynamics of the activated protein kinase C/Rack1 regulatory system in the patterning and outgrowth of limb bud mesenchyme. With minor changes, especially in the composition of the fixative solution, the protocol should be easily adaptable for the postembedding immunogold labeling of any other antigen in tissues of embryos of diverse species. Quantification of the labeling can be achieved by using electron microscope systems capable of supporting digital image analysis.

References

Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M BendayanF W Kan
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Mar 1, 1995·Cell and Tissue Research·W D SierraltaH H Thole
Oct 3, 1999·The Histochemical Journal·G R Newman, J A Hobot
Jan 1, 1958·Zeitschrift für Zellforschung und mikroskopische Anatomie·J R SOTELO, O TRUJILLO-CENOZ

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Citations

Jun 8, 2004·Plant Physiology·Michael BauerJutta Papenbrock
Dec 2, 2009·Reproductive Biology and Endocrinology : RB&E·Pedro A OrihuelaHoracio B Croxatto
Dec 12, 2003·World Journal of Gastroenterology : WJG·Feng-Peng Sun, Yu-Gang Song
Dec 8, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Felipe A CisternasMagdalena Araya
Apr 26, 2014·Biological Trace Element Research·Xuezhi WangXiaojun Kong
Mar 15, 2003·The Journal of Biological Chemistry·Kimberly M BakerMichael C Ostrowski
May 11, 2005·The American Journal of Clinical Nutrition·Magdalena ArayaBo Lönnerdal

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