Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Luane Macêdo de SousaDelane Viana Gondim

Abstract

To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ). Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1. The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections. Our results sugg...Continue Reading

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Citations

Jul 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mahmoud HashemzaeiRamin Rezaee
May 10, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lorena Vasconcelos VieiraDelane Viana Gondim
Jun 4, 2021·Inflammation·Ana Carolina de Figueiredo CostaDelane Viana Gondim
Oct 14, 2021·Cell Biology International·Zhaowei ZhangMingru Bai

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Methods Mentioned

BETA
light microscopy
nuclear translocation

Software Mentioned

SPSS
GraphPad Prism

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