PMID: 7026571Aug 1, 1981Paper

Immunofluorescence analysis of the time-course of extinction, reexpression, and activation of albumin production in rat hepatoma-mouse fibroblast heterokaryons and hybrids

The Journal of Cell Biology
M Mével-Ninio, M C Weiss

Abstract

We have used a combination of a sensitive immunocytochemical stain for intracellular albumin, and Hoechst 33258 dye for identification of parental nuclei to investigate the time-course of extinction, reexpression, and activation of albumin production in fusion products of 1s (hyperdiploid) or 2s (hypertetradiploid) rat hepatoma cells with mouse fibroblasts (L cells or embryonic cells). In all combinations, the initial event is extinction of albumin production. Extinction occurs immediately after fusion when the mouse fibroblast is a normal embryonic (senescent?) cell. In the case of an L cell, rat albumin is synthesized and secreted during the first 12 h after fusion; no production of mouse albumin occurs. Thereafter, albumin production ceases. 8-12 d after fusion, young hybrid colonies are found to resume the synthesis of rat albumin (reexpression), and several days later the production of mouse albumin begins (activation). The patterns of reexpression and activation indicate (a) that chromosome loss is not necessary for either event to occur and (b) that the cells active in the synthesis of mouse albumin are a subpopulation of those cells already engaged in the production of rat albumin. We conclude that (a) extinction is med...Continue Reading

References

Sep 1, 1978·Experimental Cell Research·L LeinwandF H Ruddle
Sep 1, 1975·The Journal of Cell Biology·F G MoserF H Ruddle
Oct 16, 1975·Differentiation; Research in Biological Diversity·C Szpirer, J Szpirer
Jun 4, 1976·Differentiation; Research in Biological Diversity·C Szpirer, J Szpirer
Feb 1, 1978·Experimental Cell Research·W E Wright, F Gros
Mar 1, 1977·Somatic Cell Genetics·J F ConscienceG J Darlington
Nov 1, 1979·Somatic Cell Genetics·J DeschatretteM C Weiss
Sep 3, 1976·Science·H Weintraub, M Groudine
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A DeisserothJ Minna
Apr 1, 1975·Somatic Cell Genetics·B P Alter, V M Ingram
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E B Thompson, T D Gelehrter
May 1, 1970·The Journal of Experimental Medicine·S Gordon, Z Cohn
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J A Peterson, M C Weiss
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S E Malawista, M C Weiss
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M C Weiss, M Chaplain
Jan 1, 1974·Annual Review of Genetics·R L Davidson
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C FougèreB Ephrussi
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R J KlebeR H Ruddle
Nov 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·R L DavidsonK Yamamoto
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L Sperling, M C Weiss
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

❮ Previous
Next ❯

Citations

Jul 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·N R CashmanJ P Antel
Apr 1, 1996·Biochemical Genetics·E B BioukarJ Deschatrette
Jan 1, 1983·Somatic Cell Genetics·T V Gopalakrishnan, J W Littlefield
Feb 1, 1984·Developmental Biology·J B Lawrence, J R Coleman
Aug 1, 1989·Trends in Genetics : TIG·H M Blau
Nov 13, 2008·Cytotechnology·Joseph J Lucas, Naohiro Terada
Dec 10, 1998·Nature Medicine·J H SimonM H Malim
Oct 13, 2007·Cell Death and Differentiation·A ConigliaroL Amicone
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M O OttM C Weiss
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D PlatikaM C Fishman
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C PetitM C Weiss
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A C Chin, R E Fournier
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A H BeggsG A Scangos
Dec 1, 1993·The Journal of Cell Biology·G IhrkeA L Hubbard
Dec 1, 1982·The Journal of Cell Biology·S LinderN R Ringertz
Sep 1, 1983·The Journal of Cell Biology·H Baumann, G P Jahreis
Dec 13, 2007·Rejuvenation Research·Alexandra StolzingSebastian Sethe
Dec 23, 2009·Progress in Biophysics and Molecular Biology·Denis Michel
Oct 24, 2007·Experimental Cell Research·Carla CicchiniMarco Tripodi
Jul 11, 2006·Journal of Cellular Physiology·Carla CicchiniMarco Tripodi
Jun 23, 2006·Journal of Cellular Biochemistry·Laura M KallayLelita T Braiterman
Jan 18, 2005·Journal of Theoretical Biology·Olivier Cinquin, Jacques Demongeot
Dec 1, 1993·Current Opinion in Cell Biology·M H Baron
Sep 1, 1986·Experimental Cell Research·M Mével-NinioR Bertolotti
Feb 5, 1985·Journal of Molecular Biology·C H SellemD Cassio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.