Immunofluorescence and flow cytometry analysis of fibronectin and laminin binding to Sporothrix schenckii yeast cells and conidia

Microbial Pathogenesis
O C LimaLeila M Lopes-Bezerra

Abstract

The adherence of Sporothrix schenckii yeast cells to several extracellular matrix (ECM) components has already been demonstrated, but the mechanisms of these interactions remained to be defined. In indirect immunofluorescence assays with polyclonal antibodies directed towards the ECM proteins, both hyphae and yeast cells of S. schenckii exhibited the ability to bind laminin and fibronectin. Flow cytometry confirmed the binding of these proteins, and revealed a significant greater binding capability for the yeast cells than for the conidia. Fibronectin and laminin binding was dose-dependent and specific. In addition, competition experiments with synthetic peptides mimicking the adhesive sequences of these proteins, or with cell wall fractions and carbohydrates constitutive of their sugar chains, were performed in order to specify the peptide or carbohydrate motifs involved in the recognition process. A 50% reduction was noticed in fibronectin binding in the presence of the synthetic peptide RGD, and a 38% reduction in laminin binding with the peptide YIGSR. Some carbohydrate-containing fractions of the yeast cell wall also inhibited the binding of fibronectin, but had no significant effect on laminin binding. Together, these res...Continue Reading

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Citations

Jul 25, 2008·Medical Mycology·Guy TronchinJean-Philippe Bouchara
Oct 7, 2011·Clinical Microbiology Reviews·Mônica Bastos de Lima BarrosArmando Oliveira Schubach
Dec 18, 2010·Future Microbiology·Everardo López-RomeroConchita Toriello
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
Aug 12, 2015·FEMS Yeast Research·Héctor M Mora-MontesLeila M Lopes-Bezerra
May 20, 2006·Anais Da Academia Brasileira De Ciências·Leila M Lopes-BezerraRosane O Costa
Mar 15, 2005·Yeast
Nov 18, 2010·Mycopathologia·Gerardo Sandoval-BernalMyrna Sabanero

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