PMID: 7029531Aug 1, 1981Paper

Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes

Proceedings of the National Academy of Sciences of the United States of America
S C FroehnerJ B Cohen

Abstract

Highly purified cholinergic postsynaptic membranes from Torpedo electric tissue contain, in addition to the acetylcholine receptor (AcChoR), major proteins of Mr 43,000 and Mr approximately 90,000 and minor proteins that can be removed from the membranes by alkaline treatment. We have prepared an antiserum to these alkaline-extractable proteins that reacts with the Mr 43,000 protein but not with any of the other major membrane proteins, including the AcChoR subunits. Immunofluorescent staining of sections of Torpedo electric tissue shows that this antiserum binds to the innervated but not the uninnervated surface of the electrocytes. In rat diaphragm muscle, the antigens recognized by this antiserum are highly concentrated at the synapse. Synaptic staining of muscle is eliminated by prior incubation of the antiserum with the Mr 43,000 protein but not by incubation with affinity-purified AcChoR. This antiserum stains end plates of muscles denervated for 7 days. Antiserum to AcChoR binds to the subsynaptic membranes of electrocytes and muscle but does not react with the Mr 43,000 protein. Purified AcChoR blocks staining of synapses by anti-AcChoR but the Mr 43,000 protein does not. These results indicate that the Mr 43,000 protei...Continue Reading

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Citations

Oct 21, 1996·The Journal of Comparative Neurology·I ColinA Triller
Nov 1, 1988·Journal of the Neurological Sciences·S KawanamiM A Raftery
Jan 1, 1988·Pharmacology & Therapeutics·F J Barrantes
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F J BarrantesT Wallimann
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A S GordonI Diamond
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H O NghiêmJ P Changeux
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S J Burden
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A S Gordon, D Milfay
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D E FrailJ P Merlie
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A HarrisG D Fischbach
Mar 1, 1986·The Journal of Cell Biology·M J Anderson
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Jul 1, 1991·The Journal of Cell Biology·S C Froehner
Oct 1, 1983·The Journal of Cell Biology·D W Pumplin, R J Bloch
Mar 15, 1983·European Journal of Biochemistry·V WitzemannC Boustead

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