Immunofluorescent localization of placental lactogen, chorionic gonadotrophin and its alpha and beta subunits in organ cultures of human placenta

Placenta
U J GaspardP Franchimont

Abstract

Localization of human placental lactogen (HPL), chorionic gonadotrophin (HCG) and its free alpha and beta subunits in mature and immature placental villi before and during organ culture was examined with an 'indirect' immunofluorescent technique using highly specific antisera. HPL fluorescence was strictly localized to the syncytiotrophoblast and the intensity of this fluorescence increased with gestational age and decreased with the time of culture. Undissociated HCG and HCG beta immunofluorescence was localized to the syncytiotrophoblast. Maximum intensity was observed in immature placentae and was not significantly affected by the duration of culture. However, irregular and patchy HCG and HCG beta immunofluorescence was seen in the cytotrophoblasts under conditions of extensive syncytiotrophoblastic damage. HCG alpha immunofluorescence was localized in the syncytiotrophoblast of immature placentae and was more intense in mature placentae. Beginning the third day of culture, HCG alpha fluorescence increased and was also present in the cytotrophoblast. On the basis of these observations and additional data, the possibility is discussed that cytotrophoblastic cells, better preserved than the syncytiotrophoblast in case of restr...Continue Reading

References

Apr 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W B Watkins
Mar 1, 1977·British Journal of Obstetrics and Gynaecology·J Hustin, U Gaspard
Dec 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R B DreskinW B Greene
Oct 1, 1966·The Journal of Pathology and Bacteriology·A R CurrieC H Read
Jun 14, 1969·Nature·P Rigby, P Curzen
Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·J VaitukaitisG T Ross
Aug 1, 1968·The Journal of Obstetrics and Gynaecology of the British Commonwealth·H Fox
Oct 1, 1966·American Journal of Obstetrics and Gynecology·R A PattilloR F Mattingly
Feb 1, 1954·Experimental Cell Research·O A TROWELL
Jun 1, 1962·The Journal of Obstetrics and Gynaecology of the British Empire·J S WIGGLESWORTH
Feb 1, 1962·The Journal of Experimental Medicine·A R MIDGLEY, G B PIERCE

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Citations

Jan 1, 1985·Virchows Archiv. A, Pathological Anatomy and Histopathology·J HustinP Franchimont
Jan 1, 1984·Archives of Gynecology·M OginoS Sakamoto
Jan 1, 1986·The International Journal of Biochemistry·B KhalfounP Bardos
Feb 1, 1987·American Journal of Obstetrics and Gynecology·K HamasakiS Fujimoto
Apr 1, 1994·American Journal of Obstetrics and Gynecology·C D HsuJ T Repke
Nov 7, 2002·Reproductive Biomedicine Online·Christoph HansisLewis C Krey
Sep 30, 2006·Reproductive Biomedicine Online·Christoph Hansis
Nov 16, 2004·Molecular and Cellular Endocrinology·M H F Sullivan
Feb 1, 1988·Biochemical Medicine and Metabolic Biology·Z HochbergR Perlman
Jun 20, 1998·The Journal of Biological Chemistry·M NemanskyD L Blithe
May 21, 2004·Reproductive Biomedicine Online·Christoph HansisLewis C Krey

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