Immunofluorescent localization of thymidylate synthase in the development of Trichinella spiralis and Caenorhabditis elegans

Molecular and Biochemical Parasitology
B GołosW Rode

Abstract

Localization of thymidylate synthase protein in Trichinella spiralis and Caenorhabditis elegans development was followed with the use of confocal microscopy, revealing similar expression patterns in both nematode species. In T. spiralis premature muscle larvae and C. elegans dauer, L3 and L4 larvae, thymidylate synthase was detected in the nerve ring and gonad primordia, as well as T. spiralis stichosome and C. elegans pharyngeal glandular cells. In developmentally arrested T. spiralis muscle larvae, the enzyme was found localized to the gonad primordia and stichosome. High enzyme level was also observed in the embryos developing in uteri of T. spiralis female adult and C. elegans hermaphrodite forms. In the case of T. spiralis adult forms, thymidylate synthase was detected in stichosome, along esophagus wall, as well as in egg and sperm cells. While the enzyme protein present in the embryos remains in accord with its known association with proliferating systems, thymidylate synthase presence in the nerve ring, and reproductive and secretory (T. spiralis stichosomal and C. elegans pharyngeal glandular cells) systems, points to a state of cell cycle-arrest, also known to preserve the enzyme protein.

References

May 16, 1997·The Journal of Biological Chemistry·W A SamsonoffF Maley
Apr 29, 1998·International Journal for Parasitology·T R BürglinM L Blaxter
May 10, 2000·Current Biology : CB·D L Myster, R J Duronio
Jun 27, 2002·Biochimica Et Biophysica Acta·Jun LiuEdward Chu
Apr 20, 2004·Cancer Cell·Lambratu RahmanMaria Zajac-Kaye
Apr 21, 2007·The Journal of Biological Chemistry·Collynn F WoellerPatrick J Stover
Dec 11, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Donald D AndersonPatrick J Stover
Jul 19, 2008·Cell Cycle·Magdalena DabrowskaWojciech Rode
Nov 7, 2008·Parasitology Research·Isao NaganoYuzo Takahashi
Nov 11, 2008·International Journal for Parasitology·David B GuilianoMurray E Selkirk
Nov 8, 2011·The Journal of Biological Chemistry·Amanda J MacFarlanePatrick J Stover

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