PMID: 7019166May 1, 1981Paper

Immunofluorescent localization of two water-soluble glycoproteins including the major allergen from the pollen of ryegrass, Lolium perenne

The Histochemical Journal
B J HowlettR B Knox

Abstract

Two major glycoproteins have been localized in sectioned grains of ryegrass pollen by direct and indirect immunofluorescence methods using Fluorescein isothiocyanate (FITC)-labelled IgG fractions of antisera. These glycoproteins are the major allergen, Group 1 allergen, and a principal antigen, Antigen A. Four methods of fixation were employed: freeze-drying, methanol, 2.5% glutaraldehyde and 4% paraformaldehyde for 1 h at 4 degrees C. The post-embedding staining technique of immunocytochemistry was used: anthers were embedded directly, or after dehydration, in JB-4 plastic resin and antibody reacted with sectioned pollen. The effects of these fixatives on the antibody combining sites of the antigens were quantified by a solid phase radioimmunoassay using [125I]protein A to measure antibody binding. In mature pollen, these antigens were located in the cytoplasm and in the complex wall. In developing grains early in the maturation period, specific fluorescence was concentrated at the periphery of the cytoplasm.

References

Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S CraigA Millerd
Jan 1, 1979·Journal of Immunological Methods·R MarierV T Andriole
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jan 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D Southworth
Jan 1, 1974·Methods in Enzymology·D M Livingston
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Nov 4, 1969·Biochimica Et Biophysica Acta·R AxenA Winter
Apr 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J A CarnegieH A Robertson
Jan 1, 1980·International Archives of Allergy and Applied Immunology·I J Smart, R B Knox

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Citations

Dec 25, 2009·Sexual Plant Reproduction·Elene R ValdiviaDaniel Cosgrove
Feb 1, 1984·The Histochemical Journal·W Van EwijkJ F Jongkind
Jul 1, 1991·Journal of Electron Microscopy Technique·L B Martinez, S M Wick

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