Immunofluorescent spectral analysis reveals the intrathecal cannabinoid agonist, AM1241, produces spinal anti-inflammatory cytokine responses in neuropathic rats exhibiting relief from allodynia.

Brain and Behavior
Jenny L WilkersonE D Milligan

Abstract

During pathological pain, the actions of the endocannabinoid system, including the cannabinoid 2 receptor (CB(2)R), leads to effective anti-allodynia and modifies a variety of spinal microglial and astrocyte responses. Here, following spinal administration of the CB(2)R compound, AM1241, we examined immunoreactive alterations in markers for activated p38 mitogen-activated protein kinase, interleukin-1β (IL-1β), the anti-inflammatory cytokine, interleukin-10 (IL-10) as well as degradative endocannabinoid enzymes, and markers for altered glial responses in neuropathic rats. In these studies, the dorsal horn of the spinal cord and dorsal root ganglia were examined. AM1241 produced profound anti-allodynia with corresponding immunoreactive levels of p38 mitogen-activated kinase, IL-1β, IL-10, the endocannabinoid enzyme monoacylglycerol lipase, and astrocyte activation markers that were similar to nonneuropathic controls. In contrast, spinal AM1241 did not suppress the increased microglial responses observed in neuropathic rats. The differences in fluorescent markers were determined within discrete anatomical regions by applying spectral analysis methods, which virtually eliminated nonspecific signal during the quantification of spec...Continue Reading

References

Feb 1, 1991·The Journal of Experimental Medicine·L Thompson-SnipesD M Rennick
Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Mar 10, 1999·Perception & Psychophysics·B Treutwein, H Strasburger
Dec 31, 2002·Chemistry and Physics of Lipids·Thien P DinhDaniele Piomelli
Jun 6, 2003·The European Journal of Neuroscience·Yuko FukudomeMasanobu Kano
Aug 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mohab M IbrahimT Philip Malan
Oct 21, 2004·The Journal of Pathology·Milos Pekny, Marcela Pekna
Oct 27, 2004·The Journal of Pain : Official Journal of the American Pain Society·Leah E SpataroLinda R Watkins
Oct 29, 2004·The American Journal of Pathology·Arianna BellucciMaria Grazia Spillantini
Mar 8, 2005·Journal of Neurochemistry·Camilla I SvenssonTony L Yaksh
Apr 22, 2005·Neuroscience Letters·Camilla I SvenssonLinda S Sorkin
May 5, 2005·The European Journal of Neuroscience·Erin D MilliganLinda R Watkins
May 26, 2005·Brain Research. Brain Research Reviews·Menachem Hanani
Oct 6, 2005·Journal of Leukocyte Biology·G A Cabral, F Marciano-Cabral
Dec 14, 2005·Journal of Neuroinflammation·Jared EhrhartR Douglas Shytle
Mar 24, 2006·The European Journal of Neuroscience·M BeltramoA Reggiani
Mar 28, 2006·Pain·Mohab M IbrahimT Philip Malan
Mar 28, 2006·Pain·Joyce A De LeoMichael L LaCroix-Fralish
Sep 7, 2006·PLoS Medicine·David L BoyleGary S Firestein
Mar 10, 2007·Protein and Peptide Letters·Balapal S Basavarajappa
Mar 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Giulio G MuccioliNephi Stella

❮ Previous
Next ❯

Citations

May 20, 2014·Expert Opinion on Investigational Drugs·Mellar P Davis
Nov 4, 2015·Journal of Medicinal Chemistry·Pushkar M KulkarniGanesh A Thakur
Jan 18, 2014·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Arjun MuralidharanMaree T Smith
Mar 23, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ellen C DenglerErin D Milligan
Dec 3, 2014·Neuropharmacology·A J KwilaszL R Watkins
Jul 18, 2015·PloS One·Seong-Jin YuYun Wang
May 10, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ivana Hradilová SvízenskáPetr Dubový
Sep 1, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Giulia DonvitoAron H Lichtman
Dec 21, 2017·Journal of Neuroinflammation·Joshua J SanchezErin D Milligan
Oct 20, 2017·Anesthesia and Analgesia·Nebojsa Nick KnezevicTony L Yaksh
May 12, 2017·Regional Anesthesia and Pain Medicine·Sheng-Jie ShiueJen-Kun Cheng
Jan 11, 2020·Frontiers in Immunology·Arden G Vanderwall, Erin D Milligan
Jun 19, 2018·Frontiers in Immunology·Shahani Noor, Erin D Milligan
Jan 23, 2016·The Journal of Pharmacology and Experimental Therapeutics·Jenny L WilkersonAron H Lichtman
May 2, 2021·Pharmacology, Biochemistry, and Behavior·Jenny L WilkersonLance R McMahon

❮ Previous
Next ❯

Methods Mentioned

BETA
spectral microscopy
transgenic

Software Mentioned

Image J
GraphPad
NIH Image J
GraphPad Prism
Nuance

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.