Abstract
Peanuts are one of the most allergenic of the foods, and hypersensitivity responses to peanut allergens can be fatal. Although the nature of the antigenic components of peanuts is being defined at the molecular level, there is little information on the induced IgE antibodies, which are central to the allergic reaction. Recognition sites of IgE antibody molecules arise from the variable regions of heavy and light chains (VH and VL). By using nested polymerase chain reactions with specific primers for the available repertoire of VH genes, together with primers in the constant epsilon region, we have amplified VH sequences of IgE from blood lymphocytes of two patients with peanut allergy. After cloning and sequencing the products, we found a predominance of VH1 family use in both patients, which was not found in control IgM-specific primers. The IgE VH sequences were highly somatically mutated, but in only six of 17 cases was there clear evidence for clustering of amino acids indicative of antigen selection. Previous results from patients with allergy to house dust mites have indicated predominance of VH5 use and little evidence for antigen selection. Although results from two patients allergic to peanuts must be regarded as preli...Continue Reading
Citations
Nov 12, 2003·Pharmacology & Therapeutics·A M CollinsM R Edwards
Dec 14, 1999·Immunology·R E SnowF K Stevenson
Sep 30, 2000·Immunology·P EibensteinerR Valenta
Mar 4, 2014·PloS One·Yan WangAndrew M Collins
Feb 12, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M Davies, R E O'Hehir
Feb 3, 2011·Scandinavian Journal of Immunology·Y WangA M Collins
Jun 25, 2010·Molecular Immunology·Katharina MarthRudolf Valenta
Sep 9, 2011·PloS One·Yvonne M VissersKarine Adel-Patient
Aug 9, 2005·The Journal of Allergy and Clinical Immunology·Heather A CokerHannah J Gould
Jul 31, 2014·Scientific Reports·Tihomir S DodevAndrew J Beavil
Mar 10, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J M Davies, R E O'Hehir
Dec 15, 2010·American Journal of Rhinology & Allergy·Eleanor PrattRichard J Harvey
Aug 16, 2006·European Journal of Immunology·Veronika PochankeKathy D McCoy
Jan 23, 2020·Journal of Leukocyte Biology·Uthaman GowthamanStephanie C Eisenbarth
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael R EdwardsAndrew M Collins
Mar 1, 2019·Science Translational Medicine·Sandra C A NielsenScott D Boyd
Jul 25, 2019·Frontiers in Immunology·Marvyn T KoningHendrik Veelken
Mar 30, 2018·Frontiers in Immunology·Wei-Li LingSamuel Ken-En Gan
Mar 8, 2011·The Journal of Allergy and Clinical Immunology·Jens HolmKaare Lund