Immunogenetic analysis of variable regions encoding AB1 and gamma-type AB2 antibodies from the NeuGc-containing ganglioside family

Hybridoma
A PerezR Perez

Abstract

The variable regions from P3, a murine monoclonal antibody (MAb) against NeuGc-containing gangliosides, and two anti-idiotype MAbs directed to P3 MAb were cloned and sequenced. Comparisons with previously reported sequences showed that P3 is a germline antibody encoded by genes from the V(H)Q52 and V(kappa)19 families. Analysis of nucleotides at the heavy chain CDR3 (H-CDR3) showed the presence of an extensive 3' N region that contains almost 50% of the nucleotides of this CDR. In addition, amino acid sequence analysis of the H-CDRs of this MAb revealed the presence of three arginines, two of which are present in the H-CDR3, that could be involved in the interaction of P3 MAb with its electronegative epitope on gangliosides. Anti-idiotype 1E10, which seems to define a "regulatory" idiotope on P3 MAb (it induces Id+ Ab3), represents a germline Ab2 that belongs to the V(H)J558 and V(kappa)10 gene families. By contrary, the anti-idiotype 3B11 is an extensively mutated antibody that belongs to the V(H)3660 and V(kappa)4/5 gene families, defining a "private" idiotope on P3 MAb. Even when different V genes contribute to the variable regions of 1E10 and 3B11 MAbs, they share an acidic motif E/D-D-Y/D-Y-D in H-CDR3, suggesting that bot...Continue Reading

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Citations

Apr 21, 2010·Immunology and Cell Biology·Victor G SendraFernando J Irazoqui
Sep 27, 2003·Hybridoma and Hybridomics·Alejandro López-RequenaAna María Vázquez
Dec 18, 2003·Hybridoma and Hybridomics·Mabel RodríguezAna María Vázquez
Apr 4, 2006·Molecular Immunology·Alejandro López-RequenaRolando Pérez
Feb 14, 2006·Journal of Neuroimmunology·Jane E LibbeyRobert S Fujinami
Jun 3, 2015·Glycobiology·Omid Haji-GhassemiStephen V Evans
Feb 3, 2007·Immunobiology·Mabel RodríguezAna María Vázquez
May 12, 2017·Journal of Immunology Research·Darel MartínezAna María Hernández
Oct 23, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Víctor BritoAna María Vázquez
Nov 26, 2014·Expert Review of Vaccines·Ana María Hernández, Ana María Vázquez
Jul 23, 2008·Molecular Cancer Therapeutics·Lourdes Roque-NavarroRolando Pérez

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