PMID: 8940981Nov 1, 1996Paper

Immunogenic and protective response in mice immunized with a purified, inactivated, Dengue-2 virus vaccine prototype made in fetal rhesus lung cells

The American Journal of Tropical Medicine and Hygiene
R PutnakC H Hoke

Abstract

The feasibility of a purified, inactivated vaccine (PIV) against dengue type 2 (DEN-2) virus was explored. Dengue-2 virus strain 16681 was used for producing a monotypic PIV. Virus adapted to fetal rhesus lung (FRhL-2) cells was harvested from roller bottle culture supernatant fluids, concentrated, and purified on sucrose gradients. Analysis of purified virus preparations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting showed primarily envelope (E) and premembrane (prM) antigens. These preparations had a purity, estimated from silver-stained gels, of approximately 70%, and a yield, based on recovery of virus and viral antigen, of 10-20%. The purified virus was inactivated with 0.05% formalin at 22 degrees C, or alternatively, with 7 mRads from a 60Co source. Vaccinated mice developed high titers of anti-DEN-2 virus neutralizing antibody and were partially protected from virus challenge. These results warrant further testing and development of PIVs for the other DEN virus serotypes.

Citations

Nov 26, 2010·Molecular Biology Reports·Muhammad FaheemIshtiaq Qadri
Apr 3, 2014·Annals of the New York Academy of Sciences·Stephen J Thomas
Nov 30, 2004·Expert Review of Anti-infective Therapy·María G GuzmánGustavo Kourí
Jun 15, 2007·Nature Reviews. Microbiology·Stephen S WhiteheadBrian R Murphy
Apr 15, 2010·Archives of Virology·Gabriela Mellado-SánchezLeticia Cedillo-Barrón
May 10, 2020·Viruses·Robert Andreata-SantosLuís Carlos de Souza Ferreira
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Aug 30, 2013·Expert Review of Vaccines·Monica A McArthurRobert Edelman
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Jan 15, 2021·Cell Host & Microbe·David R MartinezRalph S Baric
Jun 30, 2021·Expert Review of Anti-infective Therapy·Chung-Hao HuangYen-Hsu Chen
Sep 10, 2021·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Scott B Halstead

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