Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus

Vaccines
Indira S Harahap-CarrilloJorge Reyes-Del Valle

Abstract

Vaccines against dengue virus (DV) are commercially nonexistent. A subunit vaccination strategy may be of value, especially if a safe viral vector acts as biologically active adjuvant. In this paper, we focus on an immunoglobulin-like, independently folded domain III (DIII) from DV 2 envelope protein (E), which contains epitopes that elicits highly specific neutralizing antibodies. We modified the hepatitis B small surface antigen (HBsAg, S) in order to display DV 2 DIII on a virus-like particle (VLP), thus generating the hybrid antigen DIII-S. Two varieties of measles virus (MV) vectors were developed to express DIII-S. The first expresses the hybrid antigen from an additional transcription unit (ATU) and the second additionally expresses HBsAg from a separate ATU. We found that this second MV vectoring the hybrid VLPs displaying DIII-S on an unmodified HBsAg scaffold were immunogenic in MV-susceptible mice (HuCD46Ge-IFNar(k)(o)), eliciting robust neutralizing responses (averages) against MV (1:1280 NT90), hepatitis B virus (787 mIU/mL), and DV2 (1:160 NT50) in all of the tested animals. Conversely, the MV vector expressing only DIII-S induced immunity against MV alone. In summary, DV2 neutralizing responses can be generated b...Continue Reading

References

Nov 1, 1969·Journal of Virology·J L GerinR M Chanock
Aug 8, 1998·Journal of Virology·B MrkicR Cattaneo
Mar 10, 2001·Journal of Virology·B Roscic-MrkicR Cattaneo
Dec 31, 2003·Advances in Virus Research·John T Roehrig
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Feb 13, 2007·Current Opinion in Immunology·Graham R Leggatt, Ian H Frazer
Mar 18, 2011·The Journal of Allergy and Clinical Immunology·Katarzyna NiespodzianaRudolf Valenta
Feb 27, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katharina MarthRudolf Valenta
Mar 13, 2013·Protein Engineering, Design & Selection : PEDS·Nora ZidaneHugues Bedouelle
Oct 8, 2013·PLoS Pathogens·Rosa María del Angel, Jorge Reyes-del Valle
Jan 29, 2014·Trends in Microbiology·Jane P MessinaSimon I Hay
Nov 5, 2014·The New England Journal of Medicine·Luis VillarUNKNOWN CYD15 Study Group

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Citations

Nov 10, 2016·Vaccines·Kenneth Lundstrom
Sep 22, 2017·Viruses·Emily Julik, Jorge Reyes-Del Valle
Jan 1, 2019·Biotechnology and Bioengineering·Hayley K Charlton HumeLinda H L Lua
Jan 3, 2019·Expert Review of Vaccines·Christiane GerkeFrédéric Tangy
Jul 16, 2017·Virus Genes·Michael D Mühlebach
Jul 14, 2020·Frontiers in Immunology·Abdullah M IzmirlyElias K Haddad
Sep 8, 2018·Frontiers in Immunology·Nagesh K Tripathi, Ambuj Shrivastava
Jul 24, 2020·International Journal of Molecular Sciences·Kenneth Lundstrom

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Methods Mentioned

BETA
transfection
PCR
electrophoresis
ELISA
glycosylation

Clinical Trials Mentioned

NCT00866619

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