Immunogenicity and Efficacy Evaluation of Subunit Astrovirus Vaccines

Vaccines
Mehdi R M BidokhtiClaudia Baule

Abstract

A full understanding of the immune response to astrovirus (AstV) infection is required to treat and control AstV-induced gastroenteritis. Relative contributions of each arm of the immune system in restricting AstV infection remain unknown. In this study, two novel subunit AstV vaccines derived from capsid protein (CP) of mink AstV (MAstV) such as CPΔN (spanning amino acids 161-775) and CPΔC (spanning amino acids 1-621) were evaluated. Their immunogenicity and cytokine production in mice, as well as protective efficacy in mink litters via maternal immunization, were studied. Truncated CPs induced higher levels of serum anti-CP antibodies than CP, with the highest level for CPΔN. No seronegativity was detected after booster immunization with either AstV CP truncates in both mice and mink. All mink moms stayed seropositive during the entire 104-day study. Furthermore, lymphoproliferation responses and Th1/Th2 cytokine induction of mice splenocytes ex vivo re-stimulated by truncated CPs were significantly higher than those by CP, with the highest level for CPΔN. Immunization of mink moms with truncated CPs could suppress virus shedding and clinical signs in their litters during a 51-day study after challenge with a heterogeneous MA...Continue Reading

References

Dec 1, 1992·Journal of Medical Virology·G DonelliM L Marziano
May 1, 1984·Journal of Clinical Microbiology·G N WoodeJ A Fagerland
Jul 1, 1980·The Journal of General Virology·E W GrayD R Snodgrass
Jan 1, 1981·Archives of Virology·Y HoshinoF W Scott
Jul 1, 1994·The Journal of General Virology·M M WillcocksM J Carter
Oct 29, 1997·Journal of Virology·D M Bass, U Upadhyayula
Jan 22, 2000·Journal of Virology·D M Bass, S Qiu
Jan 17, 2002·Veterinary Microbiology·L EnglundK O Hedlund
Jan 19, 2002·Journal of Virology·Ute GeigenmüllerSuzanne M Matsui
Feb 22, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·B A LopmanM Koopmans
Oct 25, 2002·Avian Pathology : Journal of the W.V.P.A·Matthew D Koci, Stacey Schultz-Cherry
Apr 30, 2003·The Journal of Infectious Diseases·Stanley A Plotkin
Sep 23, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Carol O TacketMary K Estes
Oct 15, 2003·Journal of Virology·Matthew D KociStacey Schultz-Cherry
Oct 24, 2003·The Journal of General Virology·Christian MittelholzerLennart Svensson
Jan 15, 2004·Journal of Virology·Matthew D KociStacey Schultz-Cherry
Aug 13, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Dolores Gavier-WidénBerndt Klingeborn
Aug 2, 2006·Veterinary Microbiology·Stanislav IndikLadislav Rodák
May 30, 2009·Veterinary Microbiology·Anna ToffanGiovanni Cattoli
Aug 21, 2009·The Journal of General Virology·A KapoorE Delwart
Sep 18, 2009·The Journal of General Virology·Rebecca RiveraJames F X Wellehan
Nov 26, 2009·Avian Pathology : Journal of the W.V.P.A·D ToddB M Adair
May 29, 2010·Emerging Infectious Diseases·Phenix Lan QuanW Ian Lipkin
Jun 25, 2010·Journal of Clinical Microbiology·Saskia L SmitsAlbert D M E Osterhaus
Nov 12, 2010·Archives of Virology·M J Pantin-JackwoodE Spackman
May 25, 2011·Expert Review of Vaccines·Paul T Heath
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jinhui DongYizhi Tao
Aug 17, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Paola De BenedictisGiovanni Cattoli
Nov 29, 2011·Comparative Immunology, Microbiology and Infectious Diseases·R Ryan MeyerhoffMatthew D Koci
May 15, 2012·Current Opinion in Immunology·Jan Holmgren, Ann-Mari Svennerholm
Sep 7, 2012·Journal of Virology·Christine C YokoyamaLarissa B Thackray
Jul 6, 2014·Journal of Clinical Microbiology·Ilias G BouzalasTorsten Seuberlich

❮ Previous
Next ❯

Citations

Jun 20, 2020·Gut Microbes·Hyesuk SeoWeiping Zhang
Dec 5, 2020·International Journal of Biological Macromolecules·Gina M MohammedHisham A Elshoky

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
enzyme-linked immunosorbent assay
PCR
ELISA

Software Mentioned

GraphPad Prism
xPONENT

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.