Immunogenicity and efficacy of a DNA vaccine encoding a human anti-idiotype single chain antibody against nasopharyngeal carcinoma

Vaccine
Chen LuoGuancheng Li

Abstract

G22, an anti-idiotype single chain antibody screened from human nasopharyngeal carcinoma phage anti-idiotype antibody library, has been already identified by He et al. G22 DNA vaccine was produced by cloning G22 gene and inserting the cloned gene into pcDNA3.1. To investigate the immunogenicity of pcDNA3.1-G22, C57BL/6 mice were immunized with the vaccine, pcDNA3.1 and PBS individually and the antibody response, T cell phenocyte at the 15th, 22th, 29th, 36th day after the last immunity were detected. In the tumor protection experiment, the immunized mice were then challenged with CMT-93-G22 cells or CMT-93-mock cells. The tumor size and the survival time of the animals were compared between these groups. The results showed that DNA vaccine pcDNA3.1-G22 could raise G22-specific humoral and cellular immune responses. Furthermore, pcDNA3.1-G22 could prolong the survival time and lessen the tumor size of the CMT-93-G22-bearing mice but had no protection effect on the mice attacked by CMT-93-mock cells. These results were expected to lay foundation for further studies on the clinical application of pcDNA3.1-G22 DNA vaccine.

References

Feb 1, 1995·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·E OtsujiK Kitamura
Jul 22, 1999·The New England Journal of Medicine·R A Seder, S Gurunathan
Nov 9, 2000·European Journal of Immunology·L SpendloveL G Durrant
Apr 3, 2001·International Journal of Cancer. Journal International Du Cancer·R BaralM Bhattacharya-Chatterjee
Oct 24, 2001·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·T A DavisR Levy
Nov 27, 2002·Seminars in Cancer Biology·Mimi C Yu, Jian-Min Yuan
Jan 23, 2003·Cancer Biotherapy & Radiopharmaceuticals·Guancheng LiQubing Sun
Feb 21, 2003·Nature·Edith M JanssenStephen P Schoenberger
Apr 12, 2003·Science·Joseph C Sun, Michael J Bevan
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Szilvia MosolitsHåkan Mellstedt
Sep 29, 2006·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J PfistererF Hilpert

❮ Previous
Next ❯

Citations

Jan 12, 2011·International Immunopharmacology·Jia-Jia WangGuan-Cheng Li
Feb 9, 2018·Oncotarget·Liting YangZhaoyang Zeng
Jun 23, 2021·Journal of Experimental & Clinical Cancer Research : CR·Ramon YarzaLara Carmen Iglesias-Docampo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.