Immunogenicity and efficacy of immunodeficiency virus-like particles pseudotyped with the G protein of vesicular stomatitis virus

Virology
Seraphin KuateKlaus Uberla

Abstract

Vaccination with exogenous antigens such as recombinant viral proteins, immunodeficiency virus-derived whole inactivated virus particles, or virus-like particles (VLP) has generally failed to provide sufficient protection in animal models for AIDS. Pseudotyping VLPs with the vesicular stomatitis virus G protein (VSV-G), which is known to mediate entry into dendritic cells, might allow more efficient stimulation of immune responses. Therefore, we pseudotyped noninfectious immunodeficiency virus-like particles with VSV-G and carried out a preliminary screen of their immunogenicity and vaccination efficacy. Incorporation of VSV-G into HIV-1 VLPs led to hundred-fold higher antibody titers to HIV-1 Gag and enhancement of T cell responses in mice. Repeated vaccination of rhesus monkeys for 65 weeks with VSV-G pseudotyped simian immunodeficiency virus (SIV)-like particles (VLP[G]) provided initial evidence for efficient suppression of viral load after mucosal challenge with the SIVmac239 virus. Challenge of monkeys after a 28 week vaccination regimen with VLP[G] led to a reduction in peak viremia, but persistent suppression of viral load was not achieved. Due to limitations in the number of animals available for this study, improved e...Continue Reading

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Citations

Sep 28, 2007·Journal of Virology·Christiane Stahl-HennigKlaus Uberla
Oct 7, 2011·Virology Journal·Ziyaad Valley-OmarEdward P Rybicki
Feb 9, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaëlle VandermeulenGeorge Dickson
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Jul 28, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Donatella NegriAndrea Cara
Feb 21, 2018·Frontiers in Immunology·Alessandra GallinaroDonatella Negri
Jan 20, 2021·Biotechnology and Bioengineering·Jesús Lavado-GarcíaLaura Cervera
Aug 3, 2021·ACS Chemical Biology·Jianrong LiuWenhan Lin
Mar 11, 2020·Molecular Therapy. Methods & Clinical Development·Alessandra GallinaroAndrea Cara

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