Immunogenicity and immunomodulatory effects of the human chondrocytes, hChonJ

BMC Musculoskeletal Disorders
Chae-Lyul LimSujeong Kim

Abstract

Invossa™ (TissueGene-C) is a cell and gene therapy for osteoarthritis. It is composed of primary human chondrocytes (hChonJ cells) and irradiated human chondrocytes modified to express TGF-β1 (hChonJb#7 cells). The hChonJ cells were isolated from a polydactyly donor, and TGF-β1 cDNA was delivered to the cells, generating hChonJb#7 cells. Since the cells are allogeneic, the concern of immune response against cells has been raised. In this study, we investigated the immunogenicity of allogenic human chondrocyte, hChonJ cells. The immunological properties of hChonJ cells were investigated through the analysis of surface marker expression and the effect on allogeneic T cell proliferation. Flow cytometry and RT-PCR analysis were performed to analyze the surface marker expression related to immune response, such as major histocompatibility complex (MHC) class I, class II, T cell co-stimulatory molecules and T cell co-inhibitory molecules. A mixed lymphocyte reaction (MLR) was conducted to evaluate how allogeneic T cells would respond to hChonJ cells. We observed that hChonJ cells did not express MHC class II and T cell co-stimulatory molecules, but expressed T cell co-inhibitory molecule PD-L2. IFN-γ treatment induced the expression ...Continue Reading

References

Jul 27, 1999·Osteoarthritis and Cartilage·H NakamuraK Nishioka
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P HwuH A Young
Feb 27, 2001·Nature Immunology·Y LatchmanG J Freeman
Apr 3, 2001·The Journal of Experimental Medicine·S Y TsengH Tsuchiya
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomohide YamazakiHideo Yagita
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·P'ng Loke, James P Allison
Oct 28, 2003·European Journal of Immunology·Nancy RodigGordon J Freeman
Oct 2, 2004·Nature Reviews. Immunology·Andrew L Mellor, David H Munn
Jun 3, 2005·Clinical Orthopaedics and Related Research·Allan E GrossPhil Aubin
Sep 15, 2005·Biochemical and Biophysical Research Communications·Andrew Mellor
Dec 3, 2005·European Journal of Immunology·Gerhard WingenderAndreas Limmer
Feb 1, 2007·Arthritis and Rheumatism·Lazaros I Sakkas, Chris D Platsoucas
Mar 25, 2009·Clinics in Sports Medicine·Christian Lattermann, Spencer E Romine
May 17, 2011·The Journal of Rheumatology·Steven C VladDavid T Felson
Mar 18, 2015·Nature Reviews. Rheumatology·Christopher H Evans, Johnny Huard

❮ Previous
Next ❯

Citations

Dec 6, 2018·Current Stem Cell Research & Therapy·Alessandro Poggi, Maria R Zocchi
Jul 23, 2020·Molecular and Cellular Biochemistry·Ran Tang, Zhigang Xu
Mar 14, 2019·Scientific Reports·Emma CavalliMarcy Zenobi-Wong
Dec 31, 2019·Biotechnology Advances·Cui-Cui MaYu-Quan Wei
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Ilona UzielieneAli Mobasheri

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
PCR
flow cytometry
density gradient centrifugation

Software Mentioned

Rotor Gene
Sigma Plot
Macrogen
FACS DIVA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.