PMID: 9557744Apr 29, 1998Paper

Immunogenicity and protective efficacy in mice of influenza B virus vaccines grown in mammalian cells or embryonated chicken eggs

Journal of Virology
I V AlymovaR G Webster

Abstract

The immunogenicity and protective efficacy of formalin-inactivated influenza B/Memphis/1/93 virus vaccines propagated exclusively in Vero cells, MDCK cells, or embryonated chicken eggs (hereafter referred to as eggs) were investigated. Mammalian cell-grown viruses differ from the egg-grown variant at amino acid position 198 (Pro/Thr) in the hemagglutinin gene. The level of neuraminidase activity was highest in egg-grown virus, while MDCK and Vero cell-derived viruses possessed 70 and 90% less activity, respectively. After boosting, each of the vaccines induced high levels of hemagglutinin-inhibiting, neuraminidase-inhibiting, and neutralizing antibodies that provided complete protection from MDCK-grown virus challenge. Mammalian cell-derived virus vaccines induced serum antibodies that were more cross-reactive, while those induced by egg-grown virus vaccines were more specific to the homologous antigen. Enzyme-linked immunospot analysis indicated that cell-grown virus vaccines induced high frequencies of immunoglobulin G (IgG)-producing cells directed against both cell- and egg-grown virus antigens, whereas egg-grown virus vaccine induced higher frequencies of IgG- and IgM-producing cells reacting with homologous antigen and lo...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1989·The Journal of Infectious Diseases·J M Katz, R G Webster
Feb 1, 1987·Virology·J M KatzR G Webster
Dec 16, 1983·Journal of Immunological Methods·C C CzerkinskyA Tarkowski
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M KrystalP Palese
Jul 1, 1995·The Journal of Infectious Diseases·E A GovorkovaR G Webster
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·B E Johansson, E D Kilbourne
Jan 28, 1994·Cell·J Sprent
Aug 1, 1997·The Journal of Infectious Diseases·A M PalacheG J van Scharrenburg

❮ Previous
Next ❯

Citations

Jun 2, 2007·Medical Microbiology and Immunology·Jindrich CinatlHans W Doerr
Mar 27, 2009·Journal of Virology·Shinji WatanabeYoshihiro Kawaoka
Mar 20, 2012·Human Vaccines & Immunotherapeutics·Say Kong Ng
Sep 4, 2015·Nature Communications·Jihui PingYoshihiro Kawaoka
Nov 25, 2006·Biotechnology Journal·Julia Romanova
Apr 16, 2015·Human Vaccines & Immunotherapeutics·Nagendra R Hegde
May 27, 2016·The Journal of Microbiology·Jae-Min Song
Apr 8, 2020·Human Vaccines & Immunotherapeutics·Alberto Pérez-RubioJose María Eiros Bouza
Apr 20, 2018·Human Vaccines & Immunotherapeutics·Alberto Pérez Rubio, Jose María Eiros
Mar 24, 2021·Expert Review of Vaccines·Pan Ge, Ted M Ross
Oct 9, 2008·Journal of Virological Methods·Lars OpitzMichael W Wolff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.