Immunogenicity and protective efficacy of a novel recombinant BCG strain overexpressing antigens Ag85A and Ag85B.

Clinical & Developmental Immunology
C WangXionglin Fan

Abstract

Recombinant Bacillus Calmette-Guérin (rBCG) strain is the promising vaccine candidate for tuberculosis (TB) prevention, which aims at providing more enduring and enhanced protection than the parental BCG vaccine. In this study, three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed, respectively. rBCG strains showed higher level of overexpression of Ag85A and/or Ag85B proteins than BCG containing empty vector pMV261(rBCG::261), which had low levels of endogenous expression of both proteins as expected. rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses. Therefore, our results indicate that rBCG::AB could be a very promising TB vaccine candidate and should be further evaluated for the preclinical test.

References

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Citations

Jan 30, 2013·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yi-Hao DengBen-Si Zhang
Jan 22, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Charles C WangYing Zhang
Nov 7, 2013·Human Vaccines & Immunotherapeutics·Kun TanXionglin Fan
Apr 30, 2014·Frontiers in Immunology·Adeliane Castro da CostaAna Paula Junqueira-Kipnis
Jun 13, 2014·Clinical and Vaccine Immunology : CVI·Michael W Panas, Norman L Letvin

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Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA
Assay

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