Immunogenicity and Vaccine Potential of InsB, an ESAT-6-Like Antigen Identified in the Highly Virulent Mycobacterium tuberculosis Beijing K Strain

Frontiers in Microbiology
Woo Sik KimSung Jae Shin

Abstract

Our group recently identified InsB, an ESAT-6-like antigen belonging to the Mtb9.9 subfamily within the Esx family, in the Mycobacterium tuberculosis Korean Beijing strain (Mtb K) via a comparative genomic analysis with that of the reference Mtb H37Rv and characterized its immunogenicity and its induced immune response in patients with tuberculosis (TB). However, the vaccine potential of InsB has not been fully elucidated. In the present study, InsB was evaluated as a subunit vaccine in comparison with the most well-known ESAT-6 against the hypervirulent Mtb K. Mice immunized with InsB/MPL-DDA exhibited an antigen-specific IFN-γ response along with antigen-specific effector/memory T cell expansion in the lungs and spleen upon antigen restimulation. In addition, InsB immunization markedly induced multifunctional Th1-type CD4+ T cells coexpressing TNF-α, IL-2, and IFN-γ in the lungs following Mtb K challenge. Finally, we found that InsB immunization conferred long-term protection against Mtb K comparable to that conferred by ESAT-6 immunization, as evidenced by a similar level of CFU reduction in the lung and spleen and reduced lung inflammation. These results suggest that InsB may be an excellent vaccine antigen component for de...Continue Reading

References

Dec 1, 1993·The Journal of Experimental Medicine·J L FlynnB R Bloom
Dec 1, 1993·The Journal of Experimental Medicine·A M CooperI M Orme
Dec 1, 1995·Journal of Clinical Microbiology·D van SoolingenJ D van Embden
Oct 31, 1996·Annals of the New York Academy of Sciences·J L Flynn, B R Bloom
Dec 26, 1996·The New England Journal of Medicine·M J NewportM Levin
Dec 26, 1996·The New England Journal of Medicine·E JouanguyJ L Casanova
Mar 5, 1998·The New England Journal of Medicine·S E ValwayI M Onorato
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emily K ForbesElma Z Tchilian
Feb 6, 2009·Emerging Infectious Diseases·Kristin KremerDick van-Soolingen
Dec 22, 2012·Microbial Pathogenesis·Jonathan M PittAnne O'Garra
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Niels Peter H KnudsenClaus Aagaard
Jun 4, 2014·Cold Spring Harbor Perspectives in Medicine·Peter Andersen, Stefan H E Kaufmann
Nov 27, 2014·Human Vaccines & Immunotherapeutics·Daniel O VillarrealDavid B Weiner
Feb 24, 2015·Immunological Reviews·Christopher L KarpLynda M Stuart
May 8, 2016·The Journal of Infection·Yun-Gyoung HurSang-Nae Cho
Sep 27, 2016·Nature Reviews. Microbiology·Matthias I GröschelRoland Brosch
Jun 18, 2017·Frontiers in Cellular and Infection Microbiology·Zhi-Hao XiangJun Liu
Oct 20, 2017·Frontiers in Immunology·Stefan H E KaufmannUNKNOWN TBVAC2020 Consortium
Nov 9, 2017·Advances in Experimental Medicine and Biology·Joel D Ernst
Mar 24, 2018·Molecular Immunology·Preksha ShahDarren R Flower

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Citations

Aug 10, 2021·Frontiers in Public Health·Prince AsareDorothy Yeboah-Manu
Aug 6, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kristina RitterChristoph Hölscher

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Datasets Mentioned

BETA
AIB49023.1

Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA
flow cytometry

Software Mentioned

GraphPad
BLAST
FlowJo
ImageJ
GraphPad Prism

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