Immunogenicity in mice of a cationic microparticle-adsorbed plasmid DNA encoding Japanese encephalitis virus envelope protein

Vaccine
Rupinderjeet KaurSudhanshu Vrati

Abstract

Previously, we described a plasmid pMEa, synthesizing the anchored form of Japanese encephalitis virus (JEV) envelope protein that generated virus-neutralizing antibodies in mice upon intra-muscular injection. The immunized mice showed significant protection against lethal JEV challenge. In order to improve the efficacy of plasmid DNA immunization against JEV, methods need to be employed that would enhance neutralizing antibody titers. Plasmid DNA adsorbed to cationic microparticles has recently been shown to significantly improve its immunogenicity. In the present study, we have adsorbed the plasmid pMEa on cationic microparticles and have compared its immunogenicity with the naked plasmid DNA. As seen in ELISA, the microparticle-adsorbed DNA induced higher titers of anti-JEV antibodies when compared to those induced by the naked DNA. No difference, however, was seen in JEV neutralization titers. The microparticle-adsorbed DNA induced a mixed Th1-Th2 kind of immune responses as opposed to Th1 type of immune responses elicited by the naked DNA.

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Sep 11, 2008·Nature Reviews. Genetics·Michele A Kutzler, David B Weiner
Jul 21, 2005·Cancer Immunology, Immunotherapy : CII·Min Yu, Olivera J Finn
Apr 7, 2006·Expert Review of Anti-infective Therapy·Kaushik Bharati, Sudhanshu Vrati
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Aug 23, 2019·Microbial Pathogenesis·Awanish KumarKishan Kumar Nyati

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